Core--Neuropathology and tissue culture

神经病理和组织培养核心设施的核心作用是协助 本项目的目标是确定参与HIV-1脑病发展的不同分子途径。该中心将向计划项目的参与者提供来自被诊断为HIV-1脑病的HIV-1感染患者的手术和尸检脑组织材料，以及来自没有中枢神经系统参与的HIV-1感染者和正常对照大脑的大脑组织材料。在与项目1、2和3的密切合作下，我们将进行组织学和免疫组织化学分析，包括病毒蛋白、细胞标志物和各种细胞调节因子的双重标记。神经病理和组织培养核心设施将根据需要提供高度纯化的人类胎儿小胶质细胞、星形胶质细胞和神经元的培养。还将从大鼠身上制备神经元的原代培养 和小鼠海马体进行拟议的研究。已建立的细胞系PC12和P19将被维护、培养并分发给项目。此外，来自HIV-1感染细胞的条件培养液将被准备供个别项目使用。通过这一科学核心，我们将确保将必要的资源有效地分配给所有三个项目，并在保持最高水平的同时，对组织学和免疫组织化学数据进行统一评估 质量控制。

The central role of the Neuropathology and Tissue Culture Core Facility is to assist the overall objectives of this Program Project to determine the different molecular pathways involved in the development of HIV-1 Encephalopathy. The Core will furnish the participants of the Program Project with surgical and autopsy brain tissue material from HIV-1 infected patients diagnosed with HIV- 1 Encephalopathy, as well as brains from HIV-1 infected individuals with no CNS involvement and normal control brains. In close collaboration with Projects 1,2 and 3, we will perform histological and immunohistochemical analysis, including double labeling, of viral proteins, cellular markers, and various cellular regulatory factors. The Neuropathology and Tissue Culture Core Facility will provide highly purified cultures of human fetal microglia, astrocytes, and neurons on demand. Primary cultures of neurons will also be prepared from rat and mouse hippocampus for the proposed studies. The established cell lines, PC12 and P19 will be maintained, cultured, and distributed to the projects. Also, Conditioned Media from HIV-1 infected cells will be prepared for use by the individual projects. Through this scientific core, we will ensure the efficient distribution of essential resources to all three projects and uniform evaluation of histological and immunohistochemical data while maintaining the highest level of quality control.