CORE--CELL BIOLOGY

描述(摘自申请)：为…提供中央设施 浓缩细菌培养基细胞培养液的批量制备 电泳剂、琼脂平板试剂和专用维护试剂 细胞系。这一中央设施使我们能够提高质量 控制，消除以前在个人中执行的重复服务 实验室，并通过批量购买和准备介质和试剂 数量，以显著降低使用这些试剂的实验成本。 核心还作为新一批血清的试验场 验证新的一批血清支持多种细胞的生长 与DERC调查人员相关的类型，从而确保媒体质量 所有调查人员。此外，核心维护了几个专门的 产生抗体的细胞系以及产生MSA的BRL-3A细胞 [促进增殖的活动]。与膜合作 生物核心，这个核心准备和表征大量的 这些抗体和MSA供DERC成员使用。

Description (taken from application): To provide a central facility for bulk preparation of cell culture media, bacterial media, concentrated reagents for electrophoresis, agar plates and maintenance of specialized cell lines. This central facility has enabled us to enhance quality control, eliminate repetitive services previously performed in individual laboratories, and, by purchasing and preparing media and reagents in bulk quantity, to markedly reduce the cost of experiments using these reagents. The Core also serves as a test site for new lots of serum for the purpose of verifying that a new lot of serum supports growth of a variety of cell types relevant to DERC investigators, thereby ensuring media quality for all investigators. In addition, the Core maintains several specialized antibody-producing cell lines as well as BRL-3A cells which produce MSA [multiplication-stimulating activity]. In collaboration with the Membrane Biology Core, this Core prepares and characterizes bulk quantities of these antibodies and MSA for use by DERC members.