NMR of enzymic reactions and membrane transport in cells: dynamic nuclear polarization, quadrupolar relaxation, and computer modelling

该项目将使用13C快速溶解动态核偏振核磁共振波谱来研究人体红细胞中尿素转运的动力学和乙二醛酶途径，这将使13C检测能力提高10,000倍。因此，细胞过程将在一秒到四分钟的时间尺度上进行研究。此外，对133Cs+四极核的驰豫分析将探索细胞形状和膜波动的能量成本。结果将包括这些快速过程中的变化如何区分正常细胞和患病细胞，以及研究细胞的新核磁共振方法、多参数核磁共振数据分析和数学建模细胞事件以预测对物理变化和药物相互作用的反应。

This project will investigate the kinetics of urea transport and the glyoxalase pathway in human red blood cells using 13C rapid-dissolution dynamic nuclear polarisation NMR spectroscopy, which enhances 13C-detection 10,000 fold. Thus cellular processes will be studied on the one-second-to-four minute timescale. Also, relaxation analysis of the 133Cs+ quadrupolar nucleus will probe the energy cost of shape and membrane fluctuations in the cells. Outcomes will include how changes in these fast processes can distinguish normal from diseased cells, and new NMR methods for studying cells, multi-parameter NMR-data analysis, and mathematically modeling cellular events to predict responses to physical changes and drug interactions will emerge.