LC/Q Mass Spectrometry for Lipid Analysis

这项建议的目的是在南卡罗来纳医科大学(MUSC)建立一个使用质谱学的血脂分析设施。该装置的关键部件是一台LC1离子陷阱质谱仪。该仪器是由12个主要用户和4个其他用户组成的小组请求的。该用户组代表MUSC医学院的6个系。所有主要用户都有来自国家卫生研究院(NIH)的积极同行评议的研究项目赠款。即将到来的用户是最近被任命为基础科学系助理教授的，他们很有可能在获奖期开始时获得同行评审的资金。脂质分析设施的长期目标是提供尖端技术，以推进脂质第二信使领域的研究。其目标是获得一种LC-MS仪器，用于测定神经酰胺、二酰甘油(DAG)、鞘氨醇、神经鞘氨醇-1磷酸、神经酰胺-1磷酸和磷脂酸等各种关键生物活性脂质的组成和水平。其具体目的是：1.获得具有适当灵敏度的LC/Q质谱仪，用于脂类的定性和定量测定。2.提高主要用户群体成员的研究生产力。3.培养即将到来的用户群体中初级调查人员的独立性，以及他们成功竞争NIH拨款的能力。4.在不同的用户中促进和进一步发展正在进行的跨学科研究。

The purpose of this proposal is to establish a Lipid Analysis Facility using mass spectrometry at the Medical University of South Carolina (MUSC). The key component of this facility is an LC1 ion trap mass spectrometer. This instrument is requested by a group of 12 major users and 4 other users. The user group represents 6 departments of the MUSC College of Medicine. All major users have active peer-reviewed research project grants from the national Institutes of Health (NIH). The upcoming users are recently appointed assistant professors in basic science departments who have a high likelihood of obtaining peer- reviewed funding by the start of the award period. The long-term objective of the Lipid Analysis Facility is to provide cutting edge technology to advance investigations in the area of lipid second messengers. The goal is to obtain an LC-MS instrument for the determination of composition and levels of individual species of key bioactive lipids such as ceramide, diacylglycerol (DAG), sphingosine, sphingosine-1 phosphate, ceramide-1 phosphate, and phosphatidic acid. The specific aims are to: 1. Obtain an LC/Q Mass Spectrometer of appropriate sensitivity for qualitative and quantitative lipid determinations. 2. Enhance the research productivity of members of the major user group. 3. Nurture the independence of junior investigators in the upcoming user group and their ability to successfully compete for NIH grants. 4. Foster and further develop ongoing interdisciplinary research Among the various users.