NEUROMUSCULAR DEVELOPMENT AFTER BILABYRINTHECTOMY

描述：(改编自申请者摘要) 这项提案将审查是否存在发展敏感期 其中前庭感觉对于“正常”的解剖学和 体位、眼外肌和运动的生理成熟 行为。雪貂是这些研究的极好的模型，因为它出生 有着非常不成熟的感觉系统。为了识别关键时期， 两个前庭迷路将在出生后第10、15、28或60天被移除。 在行为上，雪貂的运动活动和姿势反射 记录在录像带上，并在动物发育到30岁、60岁或90岁时进行量化 几天。从生理上讲，研究人员将测量肌肉的任何变化 收缩特征，如收缩速度、力量和疲劳。 从解剖学上讲，研究人员将检查肌肉纤维类型、纤维 肌纤维的直径、类型分布及比例变化 由双侧前庭缺陷引起的肌球蛋白重链亚型。 他们的主要假设是，解剖学和行为学的变化确实发生了， 前庭感受器活动有一段敏感的时间 对于正常的神经肌肉和运动发育来说是必要的。

DESCRIPTION:(adapted from applicant's abstract) This proposal will examine whether there is a developmentally sensitive period in which vestibular sensation is necessary for the "normal" anatomical and physiological maturation of postural and extraocular muscles as well as motor behavior. The ferret is an excellent model for these studies because it is born with a very immature sensory system. In order to identify the critical period, both vestibular labyrinths will be removed at postnatal day 10, 15, 28, or 60. Behaviorally, the ferret's motor activity and postural reflexes will be recorded on videotape and quantified as the animals develop for 30, 60, or 90 days. Physiologically, the investigators will measure any changes in muscle contractile characteristics such as contraction speed, force and fatigue. Anatomically the investigators will examine changes in muscle fiber type, fiber diameter, muscle fiber type distribution and changes in the proportions of myosin heavy chain isoforms that result from bilateral vestibular deficits. Their major hypotheses are that anatomical and behavioral changes do occur and that there is a sensitive time period when vestibular receptor activity is necessary for normal neuromuscular and motor development.