课题基金 / 基金详情

CORE--TRANSGENIC AND GENE DISRUPTION LABORATORY

CORE--TRANSGENIC AND GENE DISRUPTION LABORATORY
核心——转基因和基因破坏实验室
批准号:
6595010
负责人:
GERARD C GROSVELD
金额:
$24.14万
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-06-01 至 2003-05-31

项目摘要

项目成果

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中文摘要
翻译
本核心的目的是提供转基因动物,采用转基因方法,用于项目1-4。小鼠将通过对受精卵进行DNA感染或将胚胎干细胞(ES细胞)注射到囊胚中获得。es细胞携带通过同源重组获得的靶向突变。核心接收来自不同研究人员的携带目标突变的可注射DNA或es细胞,并为其提供转基因或嵌合小鼠进行进一步繁殖。此外,该中心将协调注射,并为研究人员提供细胞系、指导和建议,以确保转基因菌株的有效产生。
英文摘要
The purpose of this core is to provide genetically modified animals, employing transgenic methodologies, for use in Projects 1-4. Mice will be obtained either via DNA infection of fertilized eggs or via injection of embryonic stem cells (ES cells) into blastocysts. The ES-cells carry targeted mutations obtained via homologous recombination. The core receives injection-ready DNA or ES-cells carrying targeted mutations from the different investigators and provide them with transgenic or chimeric mice for further breeding. In addition, the core will coordinate the injections and provide cell lines, guidance, and advice to investigators to ensure efficient generation of the transgenic strains.
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Transgenic Gene Knockout
Transgenic Gene Knockout
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CORE--TRANSGENIC/GENE KNOCKOUT SHARED RESOURCE
海外基金