CORE--EXPERIMENTAL ANIMAL FACILITY

申请者简介：实验动物核心的目标是 提供相关的小鼠模型，用于研究结肠癌的发生和发展 细胞凋亡在结肠癌发生中的作用将使用小鼠模型来评估 影响基因和细胞外因素(饮食中的多胺、胆汁酸) 细胞凋亡途径及潜在化学预防作用的评价 探员们。这些模型包括具有缺陷的APC、P53和 Nos-2基因，已经被证明或假设参与其中的基因 人类和小鼠结肠肿瘤的致癌作用。这些型号将 允许进一步澄清结肠所涉及的生化机制 并将确定更多的分子靶点来治疗癌症 化学预防。核心的具体目标是：1)提供相关的 建立小鼠模型研究结肠癌发生和细胞凋亡途径；2) 提供促进肿瘤的治疗、致癌物质和膳食补充剂 在每种适当的模式下发展；3)实施化学预防 试剂；4)提供来自动物研究方案的新鲜组织给每个 项目；以及5)对肠道肿瘤进行大体和组织学分析 在小鼠模型中发展起来。实验动物核心提供了一种 在三个不同的项目中研究所需的动物可以 共享的，研究的结果是结合的。的优势 拥有实验动物的核心是：需要的动物比每只 自己进行动物研究的项目；有一致性和 在每个项目中执行研究方案的质量控制；数据 每项研究产生的数据具有可比性，因为信息是 由相同的来源产生，具有共同的控制；核心允许 检查多种啮齿动物模型的结肠癌，否则将 买不起的或买不到的。

APPLICANT'S DESCRIPTION: The goal of the experimental animal core is to provide relevant mouse models to study colon tumor development and to study apoptosis in colon carcinogenesis. The mouse models will be used to evaluate genetic and extracellular factors (dietary polyamines, bile acids) that affect the apoptotic pathway and to evaluate the effects of potential chemopreventive agents. Included in these models are mice that have defective APC, p53, and NOS-2 genes, genes that have been shown or are hypothesized to be involved in the oncogenesis of colon tumors in both humans and mice. These models will allow further clarification of the biochemical mechanisms involved in colon carcinogenesis and will identify additional molecular targets for chemoprevention. The specific aims of the core are: 1) to provide relevant murine models to study colon oncogenesis and the apoptotic pathway; 2) to provide treatments, carcinogens, and dietary supplements that promote tumor development in each appropriate model; 3) to administer chemopreventive agents; 4) to provide fresh tissues from the animal study protocols to each project; and 5) to perform gross and histologic analyses of intestinal tumors that develop in the murine models. The experimental animal core provides a means by which animals needed for studies in three different projects can be shared and the results of the studies are combinative. The advantages to having a experimental animal core are: less animals are needed than if each project performed the animal studies by themselves; there is uniformity and quality control in carrying out the study protocols across each project; data generated from each study is comparable given that the information is generated from the same sources with common controls; and the core allows examination of multiple rodent models of colon cancer that would otherwise be unaffordable or unavailable.