CORE--CELL CULTURE LABORATORY

描述：组织培养核心是一个集中的 进行细胞培养工作并维护 标准化和质量控制。它将服务于大多数 项目调查员。该服务核心的具体目标是：1） 培养和维持专门的细胞系， 研究人员; 2）准备大量的某些细胞， 生物化学研究在个别项目;和3）提供少量 肠道细胞的其他研究人员内外 约翰霍普金斯大学。虽然核心在很大程度上是一个服务核心， 还将继续发挥更小的作用，培训调查人员使用 肠上皮细胞培养细胞。 日常工作（例如细胞系的维护、实验性 设置、内侧准备）由德怀特德尔先生执行。数量 由每个项目负责人的书面要求来定义日常工作。 这样可以协调工作，实现最大效率 并对已完成的工作进行成本核算。严格的规定已经到位 要求所有使用者遵守生物安全二级预防措施， 在设施中始终保持无菌技术。德尔先生咨询， 有必要与西尔斯博士就任何问题或出现的问题， 组织培养核心

Description: The Tissue Culture Core is intended to be a centralized service facility to perform the cell Culture work and to maintain standardization and quality control. It will serve the majority of the program investigators. The specific aims of this service core are to 1) grow and maintain specialized cell lines to be used by multiple investigators; 2) prepare large quantities of certain cells for biochemical studies in individual projects; and 3) provide small amounts of intestinal cells to other investigators both within and outside of Johns Hopkins University. Although the Core is largely a service core, it also will continue to serve a more minor role to train investigators using intestinal epithelial cells to culture cells. Daily work (e.g. maintenance of cell lines, preparation of experimental setups, medial preparation) is performed by Mr. Dwight Derr. The Quantity of daily work is defined by the written requests from each Project leader. This allows coordination of the work, achievement of maximal efficiency and cost accounting for the work performed. Strict rules are in place requiring all users to adhere to biosafety level 2 precautions and to use sterile technique at all times in the facility. Mr. Derr consults as necessary with Dr. Sears regarding any questions or problems arising in the Tissue Culture Core.