XAS COPPER CONTAINING MONOOXYGENASE ENZYMES & RELEVANT INORGANIC MODEL COMPOUNDS

多巴胺-b-羟基酶(DBH)是两种重要的单加氧酶 和肽甘氨酸羟化单加氧酶(PHM)参与 神经递质和多肽激素的生物合成，以及 因此，在细胞信号机制中具有关键的重要性，以及 神经调节功能。这项研究的重点是澄清 酶的铜(I)形式的配位化学，在 发生氧结合和底物羟化。小说 涉及CO等铜(I)特异性配体结合的方法学 异氰化物将被开发为铜(I)的有力探针 结构与取代化学。PHM的定点突变体 还将进行研究，以测试活动站点的当前模型 结构。

Two important monooxygenase enzymes, dopamine-b-hydroxylase (DBH) and peptidylglycine hydroxylating monooxygenase (PHM) are involved in the biosynthesis of neurotransmitters and peptide hormones, and are thus of pivotal importance in cell signalling mechanisms, and neuroregulatory function. The research is focussed toward elucidating the coordination chemistry of the Cu(I) forms of the enzymes, at which dioxygen-binding and substrate hydroxylation occur. Novel methodologies involving binding of Cu(I)-specific ligands such as CO and isocyanides will be developed as powerful probes of Cu(I) structure and substitution chemistry. Site directed mutants of PHM will also be studied in order to test current models of active-site structure.