Effects of AGE/RAGE on Monocytes in Arteriogenesis

描述(申请人提供)：到2010年，预计全球糖尿病患者总数将达到2.21亿人，其中高达80%的患者死于与血管疾病密切相关的并发症。形成侧支血管的能力对于补偿血管闭塞疾病引起的组织缺血至关重要，而这种能力在糖尿病患者中显著降低。 晚期糖基化终产物(AGEs)的形成发生在正常的衰老过程中，但在糖尿病的慢性高血糖状态下会增强。AGEs与糖尿病血管并发症的发生有关。 单核细胞在动脉形成过程和侧枝生长过程中发挥着重要作用，并且已知受到年龄的影响。本项目的具体目的是检测糖尿病小鼠单核细胞AGE受体的表达对微计算机断层扫描评价的后肢缺血所致动脉生成的影响，并检测AGE/RAGE刺激对上述模型所产生的单核细胞固有运动的功能影响。

DESCRIPTION (provided by applicant): By the year 2010 it is projected that the total number of people worldwide with diabetes will reach 221 million, with as many as 80% of these patients succumb to complications closely associated with vascular disease. The capacity to form collateral blood vessels is vital to compensate for tissue ischemia resulting from vascular occlusive disease, and this ability is significantly decreased in the diabetic patient. The formation of advanced glycation endproducts (AGEs) occurs in the normal process of aging, but is enhanced in the chronic hyperglycemic state of diabetes. AGEs have been implicated in the development of diabetic vascular complications. Monocytes play an important role in the arteriogenic process and during collateral growth and are known to be affected by AGEs. The specific aims of this project are to examine the effects of monocyte expression of the receptor for AGE in diabetic mice on arteriogenesis via hind-limb ischemia appraised by microcomputed tomography, and to examine the functional consequences of AGE/RAGE stimulation on the inherent motility of monocytes derived from the above model.