Crop management strategies for low asparagine grains to limit acrylamide-forming potential

项目目标：1.评估作物管理对优质小麦种质f-ASN含量的影响，氮肥和硫肥的作用，以及其他矿物质和灌溉的影响。研究不同品种间硫响应差异的分子基础。研究小麦f-ASN合成和分解的日变化规律。描述小麦籽粒中天然存在的天冬酰胺酶。对一个软质小麦作图群体进行分析，以确定f-ASN浓度的QTL。筛选3B染色体上存在/不存在f-ASN合成酶-2基因的优良品种，并评价该基因缺失对籽粒中f-ASN浓度的影响。7.量化谷物中f-ASN含量的变化与烘焙食品中AA含量的相关性

Project aims:1. Evaluate impact of crop management on f-Asn content of elite wheat germplasm, the role of nitrogen and sulphur fertilization, and the effects of other minerals and irrigation.2. Investigate the molecular basis for differential sulphur responses between varieties.3. Investigate diurnal regulation of f-Asn synthesis and breakdown in wheat.4. Characterise asparaginases naturally present in wheat grain.5. Analyse a soft wheat mapping population to identify QTL for f-Asn concentration.6. Screen elite varieties for the presence/absence of an f-Asn synthetase-2 gene on Chromosome 3B and evaluate the effect of the loss of this gene on f-Asn concentration in the grain. 7. Quantify the correlation between variation of f-Asn content in grains and AA content in baked goods