Symphony Multiplex Peptide Synthesizer w/ VISION Workstn

描述(由申请人提供)：本申请是一种带有组合反应器和专用制备型高效液相色谱仪的自动多肽合成器。我们选择了Protein Technologies Inc.的SYMPHONY Multiplex作为多肽合成器，应用生物系统公司的VISION Workstation作为制备高效液相系统。佛蒙特大学生物化学系自1984年以来一直保持着多肽合成能力。最新的仪器是CHEMTECH90型仪器，自1993年以来一直在运行，目前已到了使用寿命的尽头，支持多名调查人员的能力有限。佛蒙特大学有多名研究人员需要多肽，有了这个奖项，我们打算建立一个核心设施，主要为核心用户制造多肽。我们之所以要求SYMPHONY Multiplex，是因为它有能力满足NIH资助的多个研究项目的研究活动的需要，这些项目包括抗体开发试剂的开发、蛋白质-配体复合体的超分子组织的评估、包括蛋白质-核酸相互作用、凝血机制、肌肉功能、信号转导、抗原-抗体相互作用、氨基酸文库和新型合成酶在内的细胞调节研究。多肽合成系统将被纳入医学院生物化学系的分析设施中。财政支持将由生物化学系、医学院、佛蒙特州癌症中心和使用费提供。首席研究员Kenneth Mann博士和共同首席研究员Robert Hondal博士广泛使用多肽合成来开发抗体、研究蛋白质相互作用和蛋白质半合成。该仪器将主要由Hondal博士控制，他将由一名在使用蛋白质合成器和设施中其他分析工具方面具有专业知识的技术人员协助。此外，这一仪器设施将被纳入佛蒙特州大学生物化学、化学和其他系研究生的培训企业。该设施将使用制备型高效液相系统来提纯多肽和其他生物分子。目前，佛蒙特州大学校园内还没有一套制备型高效液相色谱系统，许多研究人员迫切需要这套系统。

DESCRIPTION (provided by applicant): This request is for an automated peptide synthesizer with a combinatorial reactor and a dedicated preparative HPLC. We have chosen the Symphony Multiplex from Protein Technologies Inc., as the peptide synthesizer and the VISION Workstation from Applied Biosystems as the preparative HPLC system. The Biochemistry Department at the University of Vermont has maintained a peptide synthesis capacity since 1984. The most recent instrument, a Chemtech model 90, has operated since 1993 is at the end of its lifetime and has limited capacity to support multiple investigators. There are multiple researchers at the University of Vermont with the need for peptides and with this award we intend to establish a core facility that would make peptides primarily for core users. We have requested the Symphony Multiplex because it has the capabilities to meet the needs of the research activities of multiple NIH funded research programs which include the development of reagents for antibody development, the evaluation of super-molecular organization of protein-ligand complexes, cellular regulation studies including protein-nucleic acid interactions, blood coagulation mechanism, muscle function, signal transduction, antigen-antibody interactions, amino acid libraries, and novel synthetic enzymes. The peptide synthesis system will be incorporated into the Department of Biochemistry's analytical facility in the College of Medicine. Financial support will be provided by the Department of Biochemistry, the College of Medicine, the Vermont Cancer Center, and by user fees. The principle investigator, Dr. Kenneth Mann and co-principle investigator Dr. Robert Hondal have used peptide synthesis extensively for antibody development, studies of protein interaction and protein semi-synthesis. This instrument will be under the primary control of Dr. Hondal who will be assisted by a technical staff with expertise in the use of the protein synthesizer and other analytical tools in the facility. In addition, this instrument facility will be incorporated into the training enterprise for graduate students in Biochemistry, Chemistry and other departments at the University of Vermont. The preparative HPLC system will be used by the facility to purify peptides and other biomolecules. There is currently no preparative HPLC system on the campus of the University of Vermont and this system is badly needed for many investigators.