Research 2 Laser Analytical Flow Cytometer

说明（由申请人提供）：本申请用于购买一台分析台式研究双激光流式细胞仪。新仪器，BD FACSCalibur，将作为研究生院公共卫生（GSPH）环境和职业卫生（EOH）系唯一的分析细胞仪，并为附近的Magee妇女研究所（MWRI）的用户提供服务，这些用户对环境健康科学和孕产妇/儿童健康方面的额外工作有共同的兴趣。这一请求的理由包括：a)环境与职业健康系（EOH）正处于广泛招聘阶段，重点是当代环境健康科学，这大大增加了我们对流式细胞术的利用。这一扩张恰逢我们的马吉妇女研究所（MWRI）最近招募了杰里·夏腾博士及其同事，从而大大进一步扩大了我们的用户群；b) EOH位于新装修的独立设施中，距离合作研究人员0.2至0.4英里（值得注意的例外是离MWRI更近），需要通过不适应环境的户外路线进行运输，最大限度地减少了共享位于我们联合医疗系统中其他几个部门的流式细胞仪的机会；c)我们的主要流式细胞仪核心设备，由Donnenberg博士领导，于2002年9月搬迁到1.5英里外的Hillman癌症中心，这增加了我们建立独立的流式细胞仪分析能力的需要；d)仪器对培养博士后和博士生至关重要；e)流式细胞仪（例如FACSCalibur）将在流式细胞仪核心咨询委员会的同事（Donnenberg博士）和生物成像中心（Watkins博士）的帮助下，提供有价值的定量方法，以支持环境健康科学中更复杂的研究方案。应用将包括多色免疫表型、转染细胞的生理研究、信号传导和凋亡、干细胞生物学和微生物学。

DESCRIPTION (provided by applicant): This request is for funds to purchase an analytical bench top research two-laser flow cytometer. The new instrument, a BD FACSCalibur, will serve as the only analytical cytometer in Department of Environmental and Occupational Health (EOH) of the Graduate School Public Health (GSPH) and for users in nearby Magee Women's Research Institute (MWRI) who share common interests in environmental health science and additional work in maternal/child health. The rationale for this request includes: a) The Department of Environmental and Occupational Health (EOH) is in an extensive recruitment phase with an emphasis on contemporary environmental health science that greatly increases our utilization of flow cytometry. This expansion coincides with the recent recruitment of Dr. Jerry Schatten and colleagues at our Magee Women's Research Institute (MWRI) thereby greatly further expanding our user base; b) EOH is in a newly renovated stand alone facility 0.2 to 0.4 miles from collaborating investigators (with the noted exception of much closer proximity to MWRI) requiring transit through unacclimatized outdoor routes minimizing the opportunity to share flow cytometers located in several other departments in our allied health system; c) Our major Flow Cytometry Core facility, directed by Dr. Donnenberg, relocated (September 2002) to the Hillman Cancer Center that is 1.5 miles away adding, to our need to establish an independent ability to perform analytical flow cytometry; d) The instrument is vital for training of pre- and postdoctoral students; and e) An analytical flow cytometer (e.g., FACSCalibur) will provide valuable quantitative approaches to support more complex research protocols in environmental health science with the aid of our colleagues on our advisory committee from Flow Cytometry Core (Dr. Donnenberg) and Center for Biological Imaging (Dr. Watkins). Applications will include multicolor immunophenotyping, physiological studies on transfected cells, signaling and apoptosis, stem cell biology and microbiology.