CORE--Molecular Virology & Gene Expression Core

分子病毒学和基因表达(MVGE)核心的目标是为CFAR研究人员提供技术支持、培训和仪器，这些技术可以被广泛的CFAR研究人员利用，他们的研究重点是分子病毒学。这一新核心的建立是我们CFAR领导层对CFAR全范围调查的直接回应，该调查表明需要支持开展与艾滋病有关的基础科学和转化研究项目的调查人员。根据调查结果，MVGE核心将为以下方面提供技术支持和培训： 1.重组蛋白在原核和真核系统中的表达和纯化 2.基于逆转录病毒和腺病毒载体的基因传递技术 3.以符合成本效益的方式获得和培训CFAR赞助的技术，如实时聚合酶链式反应 在核心实施阶段，我们首先将重点放在利用原核和真核系统为蛋白质表达和纯化提供支持服务。这些服务将在不同级别提供，从培训CFAR实验室成员到提供全面服务。在成功建立这些蛋白质表达服务后，下一个目标是为基因递送系统提供支持，例如重组逆转录病毒和腺病毒载体，这些系统通常用于不能选择转染法的应用。这些技术将通过提供个别和或小组培训和获得关键试剂来支持，目的是显著减少实验室在其研究计划中建立这些技术的时间和精力。此外，MVGE核心将提供关于CFAR设备的培训。我们将确保所有人都能轻松获得和经济高效地使用由CFAR赞助的设备 CFAR成员，并根据能力，通过(A)组织折扣试剂计划和(B)旨在最大限度地提高核心利用率的外联计划，向CWRU/UH的其他研究小组提供援助。

The goal of the Molecular Virology and Gene Expression (MVGE) core is to provide technical support, training, and instrumentation for techniques that can be utilized by a broad group of CFAR investigators whose research focus is on molecular virology. The establishment of this new core is a direct response of our CFAR leadership to a CFAR-wide survey that demonstrated the need for support of investigators performing AIDS-related basic science and translational research projects. Based on the survey results, the MVGE Core will provide technical support and training for: 1. Recombinant protein expression and purification in prokaryotic and eukaryotic systems 2. Retrovirus and Adenovirus vector-based gene deliver techniques 3. Cost-effective access to and training in CFAR sponsored technologies such as real-time PCR During the core implementation phase we will first focus on support services for protein expression and purification utilizing prokaryotic and eukaryotic systems. These services will be offered at different levels ranging from training of CFAR lab members to providing full service. After successfully establishing these protein expression services, the next goal is to provide support for gene delivery systems such as recombinant Retrovirus and Adenovirus vectors that are commonly used in applications where transfection is not an option. These techniques will be supported by providing individualized and or small group training and access to key reagents with the goal to significantly lower time and effort for laboratories to establish these techniques in their research programs. Additionally, the MVGE core will provide training on CFAR equipment. We will ensure easy access and cost-efficient usage of CFAR-sponsored equipment to all CFAR members and, based on capacity, to other CWRU/UH research groups by (a) organizing a discounted reagent program and (b) by an outreach program designed to maximize core utilization.