BEHAVIORAL AND PHYSIOLOGIC PATHOBIOLOGY OF MICE

描述(申请人提供)：首席调查员(PI)的长期职业目标是：1)继续以有意义的方式为睡眠机制和功能的基础研究做出贡献；2)增加资源和培训，以支持SIU医学院生物医学研究的持续发展；以及3)倡导人道和明智地使用动物模型来加速阐明人类疾病的原因、治疗、治疗和预防的价值和必要性。PI过去和现在的服务和行政职责限制了可用于将新的研究方法引入实验室、指导学生和其他受训人员以及作为老鼠病理生物学专家积极参与合作研究的时间。该奖项提供的机构服务的解脱将使PI有时间实现以下具体目标：1)开发新的分析(微阵列技术)和行为学[睡眠剥夺(SD)、抑郁和焦虑]方法方面的技能，应用于描绘睡眠的免疫调节作用的兴趣；2)增加针对学生和教师的指导工作；以及3)发起合作，促进和支持人类疾病的小鼠模型的研究。 这项提案的研究目标集中在确定睡眠不足对健康的影响。嗜睡和睡眠质量差广泛影响对一般健康状况的衡量，特别是影响对精力、疲劳和自给自足的看法。睡眠碎片、非恢复性睡眠和睡眠不足通常被认为会对宿主的防御能力产生不利影响。然而，这些关系还没有得到经验上的定义。在动物身上进行的一些研究表明，睡眠不足或质量不佳可能会增加疾病的易感性，加剧疾病症状，或推迟康复。开发和使用合适的动物模型来评估睡眠、睡眠缺失与感染性或炎症性疾病的易感性或康复之间的关系是这一应用的主要研究目标。

DESCRIPTION (provided by applicant): The Principal Investigator's (PI) long-term career objectives are to: 1) continue to contribute in a meaningful way to basic research on the mechanisms and functions of sleep; 2) enhance resources and training that will support the continued growth of biomedical research at the SIU School of Medicine; and 3) advocate the value of and need for the humane and judicious use of animal models to expedite the elucidation of causes, treatments, cures, and prevention of human disease. The PI's past and current service and administrative responsibilities has limited the time available for introducing new research methodology into the laboratory, for mentoring students and other trainees, and for actively participating in collaborative research as a specialist in mouse pathobiology. The relief from institutional service offered by this award will allow the PI the time to accomplish the following Specific Aims: 1) to develop skills in new analytic (microarray technology) and behavioral [sleep deprivation (SD), depression, and anxiety] methodologies for application to interests in delineating the immune modulatory role of sleep; 2) to increase mentoring efforts directed toward both students and faculty; and 3) to initiate collaborations that will promote and support the study of mouse models of human disease. Research goals for this proposal focus on defining the health implications of inadequate sleep. Sleepiness and poor sleep quality broadly influence measures of general health status, particularly impacting perceptions about energy, fatigue, and self-sufficiency. Sleep fragmentation, non-restorative sleep, and inadequate sleep are commonly assumed to adversely impact host defense capabilities. However, these relationships have not been empirically defined. Some studies in animals suggest that inadequate or poor-quality sleep may increase susceptibility to disease, exacerbate symptoms of disease, or delay recuperation. The development and use of an appropriate animal model to evaluate the relationships between sleep, sleep loss, and susceptibility to or recuperation from infectious or inflammatory disease is the primary research goal of this application.