Optical Intrinsic Signal Imaging of Seizure

描述(由申请人提供)：建筑和建筑的发展 在过去的十年里，功能神经成像技术取得了显著进展。 然而，功能神经成像技术在癫痫中的临床应用 由于它们的时间和空间分辨率不令人满意而变得复杂。 本征信号光学成像(OIS)是一种功能神经成像 测量皮质反射率随毫秒时间变化的技术 分辨率和微米空间分辨率。这些光学图像是相关的 与神经元活动有关，是由于脑血容量的变化，轻 散射，并处于血红蛋白和细胞色素氧化状态。在 初步研究，OIS是从大鼠的躯体感觉皮层记录到的， 近红外频率(850 Nm)。OIS被证明与癫痫发作有很好的相关性 活动，以可恢复和可复制的方式。还有人指出， OIS的变化可以在最初的脑电波尖峰出现之前长达1分钟。目标是 该项目的目的是将多波长光信号与 电生理和免疫组织化学标志物，并建立一种 解释OIS癫痫数据的基本基础。这样做的结果 该项目可在未来应用于各种癫痫模型，如在 大脑皮层点燃模型在神经网络行为研究中的应用 点火过程。OIS成像可能具有更好的前景 对癫痫的发生、诱发、停止和传播的认识 小路。OIS成像在癫痫手术中的附加应用 定义口才皮层并确认癫痫发作的焦点应该是 在未来彻底探索。候选人陈医生是一名神经科医生 以及一位擅长使用膜片钳的细胞电生理学家 技术。他的职业目标是通过以下方式阐明癫痫的基本机制 整合他已经学到或将学到的所有研究技能 电生理学、光学成像、神经化学和计算机模拟。 该项目将在加州大学洛杉矶分校医学院由Toga博士领导 以及韦斯特伦的指导。两位导师都是国际知名的权威人士 在他们各自的研究领域。

DESCRIPTION (provided by applicant): The development of both structural and functional neuroimaging has progressed significantly in the last decade. However, clinical application of functional neuroimaging techniques in epilepsy has been complicated by their unsatisfactory temporal and spatial resolution. Optical imaging of intrinsic signals (OIS) is a functional neuroimaging technique that measures cortical reflectance changes with millisecond temporal resolution and micron spatial resolution. These optical images are correlated with neuronal activity and are due to changes in cerebral blood volume, light scattering, and in hemoglobin and cytochrome oxidation state. In the preliminary studies, OIS was recorded from the somatosensory cortex of rats at, near infrared frequency (850 nm). OIS was shown to correlate well with seizure activities, in a recoverable and reproducible fashion. It was also noted that OIS changes could precede the initial EEG spikes for up to I minute. The goal of this project is to correlate multiplewavelength optical signals with both electrophysiological and immunohistochemical markers, and to establish a primary foundation of interpreting OIS seizure data. The results of this project can be applied in the future in various epilepsy models, such as in a cortical kindling model for the study of neuronal network behaviors during kindling process. OIS imaging might hold a promising potential of better understanding of epileptogenesis, seizure induction, cessation and propagation pathways. Additional application of OIS imaging during epilepsy surgery in defining the eloquent cortex and confirming the seizure focus should be explored thoroughly in the future. The candidate, Dr. Chen, is a neurologist and a cellular electrophysiologist who has the expertise of using patch clamp technique. His career goal is to elucidate the basic mechanisms of epilepsy by integrating all the research skills that he has learned or will learn in electrophysiology, optical imaging, neurochemistry and computer simulation. This project will be conducted at the UCLA School of Medicine under Drs. Toga and Wasterlain's guidance. Both mentors are internationally known authorities in their respective fields of research.