Gene Expression Profiling Core

受感染的宿主细胞对有害的细胞内病原体的动态反应是自然历史和感染结果的关键决定因素。这些反应既反映并导致基因表达的变化，可以为宿主-病原体相互作用的核心途径和过程提供分子洞察力。因此，对项目1-4的研究至关重要的是一组资源，用于比较宿主巨噬细胞对不同细胞内病原体及其分子决定因素的全基因组表达谱。本提案的目的是提供必要的资源和工具，用于生成和比较小鼠巨噬细胞对一组细胞内病原体（结核分枝杆菌、荚膜组织胞浆菌、土拉弗朗西斯菌和单核增生李斯特菌，分别对应于项目1-4）的基因组级表达反应。这些

The dynamic responses of infected host cells to an offending intracellular pathogen are a pivotal determinant of the natural history and outcome of infection. These responses both reflect and result in gene expression changes that can provide molecular insight into the pathways and processes central to the host-pathogen interaction. Therefore, essential to the studies of Projects 1-4 are a set of resources for comparing the genome-wide expression profiles of host macrophages responding to distinct intracellular pathogens and their molecular determinants. The aim of this proposal is to provide the necessary resources and tools required for generating and comparing genome-scale expression responses of murine macrophages responding to a group of intracellular pathogens (Mycobacterium tuberculosis, Histoplasma capsulatum, Francisella tularensis, and Listefia monocytogenes, corresponding to Projects 1-4, respectively). These resources include the design, production, and distribution of mouse DNA microarrays based on long oligonucleotides selected using a novel strategy that combines the nearly complete mouse genome sequence with millions of mRNA and EST sequences. In addition to the use of standardized methods for generating expression profiles for these infection processes, the utility of these microarrays relies heavily on the development and maintenance of a corresponding bioinformatics infrastructure for the storage, analysis, in distribution of the bodies of data generated by the Program participants. Accordingly, Core B aims to create and maintain a secure, robust and user-friendly repository to store, retrieve, analyze, display and disseminate microarray data. Furthermore, Core B will provide statistical support for the investigators in the Program for study design and analyses, as well as train and support Program participants in the design, execution, and analysis of microarray experiments.