Core--ANIMAL, GENOTYPING, CELL & TISSUE (AGCT)

动物，基因分型，细胞和组织（AGCT）核心将为动物育种，促进，标准化和实施动物基因分型以及组织样本的收集，管理和分析提供技术和人员支持。AGCT核心设施将为所有需要以下方面帮助的研究人员提供服务：(1)动物护理、育种和免疫；(2)组织和/或体液的收集；(3)Finnegan、Zhang和Mikecz项目在标记辅助回交（“快速遗传”）过程中的所有基因分型；(4)基本细胞培养方法；(5)评估关节炎发展和进展的标准化测试。(6)对组织样品进行组织学处理和评价。AGCT核心计划通过提供技术、仪器和人员支持来帮助每个项目，这些支持是(i)几个项目通常使用的，或（ii）偶尔由调查人员使用，但需要特殊技术或安全法规，或（iii）特殊设备或技术经验。此外，AGCT核心提供基本的统计和必要的生物信息学分析。根据该应用的预期需求，AGCT核心设施将由四个单元组成。动物护理核心单元将设计并进行基因型控制育种，根据研究人员的需要对动物进行免疫，并以pi独立（盲）方式评估关节炎的进展。因此，该股将为所有项目提供生产和试验可供个别项目使用的动物的服务。基因分型核心单元服务于所有需要在回交过程中进行基因分型的项目。这包括用于基因组DNA分离的样本收集，记录保存和标记动物，以及具有

The Animal, Genotyping, Cell & Tissue (AGCT) Core will provide technical and personnel support for breeding animals, facilitating, standardizing, and implementing animal genotyping and the collection, management and analysis of tissue samples. The AGCT Core Facility will serve all investigators who will be needing assistance with (1) animal care, breeding and immunization, (2) collection of tissue and/or body fluid, (3) all genotyping for Projects ny Finnegan, Zhang, and Mikecz along the marker-assisted backcrossing ("speed congenic") processes, (4) basic cell culture methods, (5) standardized testing for assessment of the development and progression of arthritis, and (6) processing samples for histology and evaluation of tissue samples. The AGCT Core intends to aid each project by providing technical, instrumental and personnel support which are (i) commonly used by several projects or (ii) occasionally used by investigators but require special technical or safety regulations or (iii) special equipment or technical experience. In addition, the AGCT Core offers basic statistical and necessary bioinformatic analysis. The AGCT Core facility, based on the expected demands of this application, will consist of four units. The Animal Care Core Unit will design and perform genotype-controlled breeding, immunize animals according to the needs of the investigators and assess the progression of arthritis in PI-independent (blind) manner. Thus, this Unit will serve all projects generating and testing animals ready for use by individual projects. The Genotyping Core Unit serves all projects needing genotyping along the backcrossing processes. This includes sample collection for genomic DNA isolation, record keeping and labeling animals, and genotyping of animals with strain-specific polymorphic markers. Due to the large number of animals and multiple congenic/sub-congenic lines, the marker-assisted breeding process of Project by Glant and selection of animals with appropriate (required) recombinations does not belong to this core. On the other hand, the same person (director of this core and co-investigator of Project by Glant ) will control all animal breeding and genotyping experiments. The Cell and Tissue Testing Core Unit will support all investigators by collecting animal tissues and human cartilage, preparing antigens for immunization, and tissue and serum samples for standardized testing. This unit will be responsible for sample storage, record keeping and data management of collected samples, and evaluate/score histological specimens. This core unit will help investigators collect cells or tissues for specialized analysis (e.g. peripheral blood, spleen cells, or establish primary cell cultures), perform histology and/or genotyping tests of cells, and store tissue samples for further analyses. The BiostatisticallBioinformatics Core Unit will standardize and perform statistical analysis and support investigators in data management.