Animal care: supporting research on pathogenesis and treatment of autoimmunity

Lacu协助NIAMS IRP调查人员进行动物研究。这些动物被安置在NIH园区的共享动物设施中，包括位于ACRF 6B、10A、50号楼和14号楼综合体中10号楼9/C127的NIAMS Lacu直接监督设施。NIAMS的动物使用者主要与小鼠和大鼠合作。 NIAMS IRP动物计划监督了18项使用小鼠和大鼠模型的动物研究建议(ASP)。LACU协助研究人员准备ASP，并在NIAMS动物保护和使用委员会(ACUC)拥有2名投票成员和1名候补成员，并维护IRP的寄养群体和哨兵计划。项目包括关节炎、过敏性鼻炎和其他由传递受体诱导的细胞反应、使用AID转基因小鼠进行重组/染色质重塑、家族性地中海热(FMF)和肿瘤坏死因子受体相关的周期性综合征。研究涉及肌肉和相关疾病(实验：N-RAP蛋白、糖原储存疾病II型、SIRT1基因、肌原纤维组装和肌炎)和骨骼肌亚细胞细胞器和细胞骨架，以及利用胎儿和成人皮肤组织进行逆转录病毒转导、骨髓移植和表皮分化的体外实验。许多研究所的研究涉及细胞凋亡和自身免疫，以及软骨研究，重点是组织工程软骨、软骨生成、GDF-F、COMP和骨关节炎。NIAMS IRP LACU在2011年6月的计划审查和检查期间，为NIH与国际实验动物护理评估和认可协会(AALACi)的认证提供了便利。2011年6月，实地考察取得了成功，特别是对动物项目、ACUC和研究人员在认真和人道地处理研究动物方面所表现出的合作伙伴关系的赞扬。没有直接归因于NIAMS的遵从性缺陷。下一次检查将在2014年初夏进行。10/9C127动物设施目前正处于翻新的最后阶段，我们预计将在现有设施的基础上增加700至900个老鼠笼。

The LACU assists the NIAMS IRP investigators with their research involving animals. Such animals are housed in shared animal facilities on the NIH campus including the NIAMS LACU directly overseen facility located in Building 10 in 9/C127 in the ACRF Buildings 6B, 10A, 50 and the Building 14 complex. NIAMS animal users work primarily with mice and rats. The NIAMS IRP Animal Program has oversight for 18 Animal Study Proposals (ASPs) using mouse and rat models. The LACU assists researchers prepare ASPs and has 2 voting members and 1 alternate on the NIAMS Animal Care and Use Committee (ACUC) and maintains the foster colony and the sentinel program for the IRP. Projects include arthritis, allergic rhinitis and other transmit receptor-induced cellular responses, recombination/chromatin remodeling using AID transgenic mice, familial Mediterranean fever (FMF), and TNF receptor associated periodic syndrome. Research involves muscle and related diseases (experimentation with: N-RAP protein, glycogen storage disease Type II, SIRT1 gene, myofibril assembly, and myositis) and sub-cellular organelles and cytoskeleton in skeletal muscle as well as in vitro experiments examining retroviral transduction, bone marrow transplantation, and epidermal differentiation using fetal and adult skin tissues. Many of the institutes studies involve apoptosis and autoimmunity, as well as cartilage studies focused on tissue-engineered cartilage, chondrogenesis, GDF-F, COMP and osteoarthritis. The NIAMS IRP LACU facilitated the accreditation of NIH with the Association for the Assessment and Accreditation of Laboratory Animal Care International (AALACi) during the program review and inspection in June of 2011. June 2011 site visit was successful with particular commendation to the demonstrated partnership of the Animal Program, ACUC, and investigators in dealing conscientiously and humanely with research animals. No compliance deficiencies were directly attributed to NIAMS. The next inspection will be in early summer of 2014. The 10/9C127 Animal Facility is currently in the final stages of renovation and we expect to add between 700 and 900 mouse cages to the existing facility.