Clinical Information

抗体开发设施为研究人员提供定制设计的单抗 抗体。在大多数情况下，即使不是所有情况下，这些都是商业上无法获得的必要试剂。提供的服务包括：a)设计抗原、免疫和筛选策略b)通过传统方法生产杂交瘤细胞系，c)通过选择克隆优化现有的杂交瘤细胞系d)在小鼠以外的物种中生产单抗，e)使杂交瘤细胞系脱离选择介质，进入各种基础和无血清的介质中，用于微型生物反应器、免疫沉淀或体内研究， F)单链抗体可变区(ScFv)或含有绿色荧光蛋白等生物报告分子的单链抗体可变区(ScFv)的克隆、表达和工程；这项申请要求继续为继续履行同行评议研究的基本作用的资源提供支持。

The Antibody Development Facility provides investigators with custom designed monoclonal antibodies. In most, if not all cases, these are necessary reagents that are not commercially available. Services provided include: a) the design of antigens, immunization and screening strategies b) hybridoma cell line production via traditional methods, c) optimization of existing hybridoma cell lines by selection cloning d) production of monoclonal antibodies in species other than mice, e) services to wean hybridoma cell lines off selection media and into a variety of basal and serum free media for use in mini-bioreactors, immune precipitation or in vivo studies, f) cloning, expression and engineering of single chain antibody variable fragments (scFvs) or scFvs containing biological reporter molecules such as green fluorescent protein and g) the selection of antigen specific antibodies and their fragments from phage display libraries. This application requests continued support for a resource which continues to fulfill an essential role for peer reviewed research.