Gene Expression Profiling, Insulin Resistance, & Obesity

我们进行了全球基因表达谱分析，以补充正在进行的定位克隆工作，以确定糖尿病及其预测因子、胰岛素抵抗和肥胖的易感基因。为此，我们使用包含6800个基因和35000个表达序列标签（est）的人类寡核苷酸微阵列检测了非糖尿病纯血统皮马印第安人组织样本的基因表达谱。

We performed global gene expression profiling to complement the ongoing positional cloning effort to identify susceptibility genes for diabetes and its predictors, insulin resistance and obesity. To this end, we have examined gene expression profiles in tissue samples from non-diabetic full-blooded Pima Indians using human oligonucleotide microarrays containing 6800 genes and 35000 expressed sequence tags (ESTs). To identify possible determinants of insulin resistance, independent of the effects of obesity, differentially expressed genes were identified in skeletal muscle samples from a group of equally obese Pimas, matched for obesity, age and sex. To identify possible determinants of obesity, we identified differentially expressed genes in isloated, subcutaneous, abdominal adipocytes from a group of severly obese and lean Pimas matched for age and sex. To identify genes that were potentially secondarily differentially expressed, (e.g. as a result of hyperinsulinemia), we also determined leveles of gene expression in pre-adipocytes grown from these same adipose tissue samples.