Sequencing the Dictyostelium genome

描述（由申请人提供）：进化保护 蛋白质的结构-功能关系使我们能够整合信息 从不同的物种，并作出推论的生物现象， 不同的生物体。在接下来的十年里， 大多数基因将获得几个模式生物。Dictyosteroids是一种 社会变形虫，其研究有助于我们了解不同的 许多真核生物共有的生物过程。模型的深入研究 像网骨藻这样的生物将告诉我们人类的生物学， 将不可避免地有助于疾病的检测和治疗。 该提案描述了一个持续的努力，以测序约一半的 网骨藻基因组。上一个项目期侧重于可行性 研究和6号染色体的测序。这项提案针对4号染色体 和5，它们一起构成了34-Mb基因组的约三分之一。我们将 使用全染色体鸟枪测序与样品测序相结合， 大插入克隆的最小重叠集，以生成连续和 高度精确的序列。组装好的DNA将与一个 高分辨率物理图，根据这些图规划最后的实验 并执行以完成序列。序列将被丰富地注释 并提交给公共数据库。将提供所有原始序列数据 尽快通过我们的网站。 完整基因组序列的获得有望加速研究 细胞和发育生物学的基本机制， 现在的任务是定义这些基因组中包含的基因， 功能适合于高通量方法的微生物 诸如网骨藻等研究已经并将继续 在这一奋进中发挥作用。网骨藻基因组序列将提供 第一次详细地了解了一个生物系统， 单细胞生物，但仍然简单到足以完全理解， 可预见的未来基因组数据将使所有领域的专家， 生物学丰富的初步发现，并作出有益的推断，以其他 物种完整的序列数据从实验上容易处理的 生物，如网囊藻，将继续刺激基因组 包括人类在内的许多系统中的调查。

DESCRIPTION (provided by applicant): Evolutionary conservation of structure-function relationships in proteins allows us to integrate information from various species and make inferences about biological phenomena in different organisms. Over the next decade a basic understanding of the function of most genes will be obtained for several model organisms. Dictyostelium is a social amoeba whose study has contributed to our understanding of diverse biological processes common to many eukaryotes. The in-depth study of model organisms such as Dictyostelium will inform us about human biology and this will inevitably aid in the detection and treatment of disease. This proposal describes a continuing effort to sequencing about one-half of the Dictyostelium genome. The previous project period focused on feasibility studies and the sequencing of chromosome 6. This proposal targets chromosomes 4 and 5 which together constitute about one-third of the 34-Mb genome. We will use whole chromosome shotgun sequencing combined with sample sequencing of a minimum overlapping set of large-insert clones to generate contiguous and highly accurate sequence. The assembled DNA will be integrated with a high-resolution physical map from which finishing experiments will be planned and carried out to complete the sequence. The sequence will be richly annotated and submitted to public databases. All raw sequence data will be made available as soon as possible via our website. The availability of complete genome sequences promises to accelerate research into fundamental mechanisms of cell and developmental biology and the challenge now is to define the genes contained in those genomes and determine their function. Microorganisms that are amenable to high-throughput methods of investigation such as Dictyostelium have been and will continue to be instrumental in this endeavor. The Dictyostelium genome sequence will provide the first detailed glimpse of a biological system that is more complex than single-celled organisms, yet still simple enough to understand completely in the foreseeable future. The genomic data will allow specialists in all areas of biology to enrich the initial findings and make useful extrapolations to other species. Complete sequence data obtained from experimentally tractable organisms, such as Dictyostelium, will continue to stimulate genomic investigations in many systems, including humans.