Measurement Of Cytokines In Cervical Fluids

疱疹病毒和肿瘤之间的关系已经有了很好的记录，尽管宿主因素在这种关系中所起的作用仍然是未知的。该项目的主要目的是确定在感染肿瘤类型的人乳头瘤病毒（HPV）的妇女的宫颈标本中测量的多分析物细胞因子谱是否可以预测随后进展为高级别宫颈上皮内瘤变（CIN）的风险。使用一种称为再循环免疫亲和层析（RIC）的技术，检查宫颈液中的细胞因子谱，以确定哪些细胞因子和其他因子在肿瘤过程中升高或激活。将采用30个分析物组比较高级别CIN女性样本与致癌HPV感染女性样本和正常对照样本中的细胞因子谱。通过这些研究，希望确定哪些细胞因子或其他因子是预测肿瘤进展的候选者。一旦确定，这些标志物将用于HPV和CIN自然史的前瞻性研究。最初的研究证明了RIC系统的有效性，并表明细胞因子面板可用于确定不同的患者组。进一步的研究表明，RIC系统是可靠的和可重复的。目前正在将这一分析系统应用于流行病学实地研究。

The relationship between Herpes viruses and neoplasia has been quite well documented although the role played by host factors in this relationship is still unknown. The prime objective of this project to determine whether multi-analyte cytokine profiles measured in cervical specimens, from women infected with oncological types of Human Papillomavirus (HPV), can predict risk of subsequent progression to high-grade cervical intraepithelial neoplasia (CIN). Using a technique called recycling immunoaffinity chromatography (RIC), examination of cytokine profiles in cervical fluid will be performed to determine which cytokines and other factors appear to be elevated or activated during the neoplastic process. 30 analyte panels will be employed to compare cytokine profiles in samples from women with high-grade CIN to samples from women with oncogenic HPV infection and normal controls. From such studies it is hoped to determine which cytokines or other factors are candidates for predicting neoplastic progression. Once this has been established these markers will be used in a prospective study of HPV and CIN natural history. The initial studies demonstrated the efficacy of the RIC system and have shown that cytokine panels are useful in determining the different patient groups. Further studies have demonstrated the RIC system to be reliable and reproducible. Work is now progressing to apply this analytical system to epidemiological field studies.