Novel Amino Acid-Based Glass-Ionomer Biomaterials

描述（由申请人提供）：众所周知，树脂改性玻璃离子水泥（RMGIC）是一种有吸引力的牙科修复材料，因为它具有增强的机械强度，粘接和处理性能。然而，这种水泥的缺点是它含有大量的2-羟乙基甲基丙烯酸酯（HEMA）。由于未反应的HEMA对牙髓和周围组织具有细胞毒性，因此消除HEMA可以使这种“智能”水泥在牙科和骨科应用中更具吸引力。我们已经证明，新的和新的氨基酸修饰和不含hema的rmgic具有显着提高的机械强度，附着力和自蚀刻能力。这项研究的目的是开发这些新型的基于氨基酸的玻璃离聚体，用于牙科和骨科应用，以响应NIBIB的RFA，开发具有改进生物和机械性能的新型生物材料。在本研究中，将合成廉价且生物稳定的氨基酸基多羧酸，并与氨基酸衍生物配制，形成一种创新的不含hema的RMGIC体系。采用实验设计（DOE）方法对合成的聚氨基酸的摩尔比、分子量、接枝比、配方和填料含量进行优化。将评估新水泥的机械强度和其他物理性能。提出的氨基酸基水泥将提供额外的盐桥，从而增强附着力和机械强度。将进行体外直接细胞接触、甲基噻唑基二苯四氮唑（MTT）研究和体内骨反应，以确定新型水泥的生物相容性和生物活性。牙科和骨科的新型修复体应该是这一基础研究的结果，为未来的工业发展建立商业化的生物材料。该项目的成功实现将对修复牙科和矫形外科领域产生重大影响。这个项目将在整个大学范围内建立一个合成聚合物化学家、生物材料科学家、生物力学工程师和临床牙医之间的合作。该项目还将为生物医学工程专业的学生提供一个理想的培训环境，因为它将使他们通过与不同学科的合作者的密切互动，发展生物材料研究的广阔视野。

DESCRIPTION (provided by applicant): It is known that resin-modified glass-ionomer cement (RMGIC) is an attractive dental restorative because it has enhanced mechanical strength, bonding and handling properties. However, a disadvantage of this cement is that it contains a large quantity of 2-hydroxyethyl methacrylate (HEMA). Since unreacted HEMA is cytotoxic to pulp and surrounding tissues, elimination of HEMA could make this "intelligent" cement more attractive for both dental and orthopedic applications. We have demonstrated that new and novel amino acid-modified and non-HEMA containing RMGICs exhibit significantly improved mechanical strengths, adhesion and self-etching capabilities. The objective of this research is to develop these novel amino acid based glass-ionomers for both dental and orthopedic applications, in response to NIBIB's RFA for development of novel biomaterials with improved biological and mechanical properties. In this research, inexpensive and biostable amino acid-based polycarboxylic acid will be synthesized and formulated with amino acid derivatives to form an innovative non-HEMA containing RMGIC system. Design of Experiment (DOE) methodology will be applied to optimize molar ratio, molecular weight, grafting ratio of synthesized poly(amino acid), formulations and filler contents. Mechanical strengths and other physical properties of the new cements will be evaluated. The proposed amino acid-based cements will provide extra salt-bridges, thus enhancing adhesion and mechanical strengths. In vitro direct cell contact as well as methylthiazolyldiphenyl tetrazolium (MTT) studies and in vivo bone response will be conducted to determine the biocompatibility and bioactivity of the new cements. Novel restoratives for both dentistry and orthopedics should be an outcome of this basic research to establish commercial biomaterials for future industrial development. Successful achievement of the goal of this project will significantly impact the field of restorative dentistry and orthopedic surgery. This project will create a university-wide collaboration between synthetic polymer chemist, biomaterials scientist, biomechanical engineer and clinical dentist. This project will also provide an ideal training environment for biomedical engineering students because it will allow them, through close interactions with the collaborators from different disciplines, to develop a broad perspective on biomaterials research.