Multiplex Fluorescent Dyes for Ultrasensitive Proteomics

描述（由申请人提供）：该STTR提案描述了Zdye LLC和蒙大拿州立大学（MSU）之间的合作研究，以合成和展示用于蛋白质组学中的多重（多重）检测的创新超灵敏荧光染料。所得到的“Z染料”被设计用于极大地改进差异蛋白质表达的定量和蛋白质翻译后修饰的变化。蛋白质组学是生物技术的一个主要前沿，因为蛋白质在细胞中完成大部分工作，并且蛋白质活性在很大程度上受翻译后修饰控制。虽然蛋白质组学在揭示生物学机制方面具有巨大的潜力，但目前的方法无法实现这一承诺。Zdyes将提供比当前技术至少百倍的灵敏度增加，用于在2D凝胶上检测。第一阶段将建立Zdye的可行性，以提高回收率，通过质谱法增强蛋白质和蛋白质修饰的鉴定，并通过三色通道定量蛋白质表达率。第二阶段将进行β测试，并纳入额外的染料颜色通道，以同时监测蛋白质翻译后修饰。Zdye/MSU研究团队拥有广泛的相关专业知识，Zdye准备生产和商业化由此产生的产品。Zdyes将对几乎所有健康领域的诊断开发和研究产生直接的积极影响，包括预防医学，2型糖尿病，心脏病和癌症。

DESCRIPTION (provided by applicant): This STTR proposal describes collaborative research between Zdye LLC and Montana State University (MSU) to synthesize and demonstrate innovative ultrasensitive, fluorescent dyes for multicolor (multiplex) detection in proteomics. The resulting "Zdyes" are designed for vastly improved quantitation of differential protein expression and changes in protein post-translational modifications. Proteomics is a major frontier in biotechnology as proteins do most of the work in cells and protein activity is largely controlled by posttranslational modifications. While proteomics has enormous potential for revealing biological mechanisms, current methods fall short of fulfilling this promise. Zdyes will provide at least a hundred fold sensitivity increase over current technology for detection on 2D gels. Phase I will establish Zdye feasibility to improve recovery for enhanced identification of proteins and protein modifications by mass spectrometry, and for quantitative protein expression ratios with three-color channels. Phase II will carry out beta testing, and incorporate additional dye color channels to simultaneously monitor protein post-translational modifications. The Zdye/MSU research team has extensive relevant expertise and Zdye is prepared to produce and commercialize the resulting products. Zdyes will have immediate positive impact on diagnostic development and research in virtually all health fields, including preventative medicine, type 2 diabetes, heart disease and cancer.