Development of Peromyscus Genomics

描述(申请人提供)：Permyscines是分布最广、数量最多的北美本土哺乳动物。近年来，白斑拟青霉和黑线拟青霉被发现是引起莱姆病和汉坦病毒肺综合征两种新发传染病的主要微生物储存库，引起了人们的广泛关注。被恰当地称为北美哺乳动物学中的果蝇，霉菌也被认为是研究1)负责生殖隔离和物种形成的基因，2)使生理和行为适应不断变化的环境条件，适应其他物种，相互适应，以及适应微生物和其他寄生虫的理想模型。 开发这一潜力的一个重要工具是连锁图谱。这项建议的目的是利用基于聚合酶链式反应的标记，在足够的密度下，大约5-10 cM，开发一个贝氏小蜂的中等密度连锁图谱，以便能够识别与参考物种小鼠共线的主要片段。这些标记包括1)类型I(蛋白质编码基因)和2)类型II(微卫星)，类型I(蛋白质编码基因)对共性鉴定很重要，类型II(微卫星)具有高度多态，最终将用于QTL分析。连锁分析小组是为了最大限度地利用多态而选择的，它由姊妹种P m bairdii和小儿麻痹症患者杂交的种间减数分裂片段组成。 Permyscus连锁图谱的开发将显著提高该物种的研究潜力，加强目前正在进行的项目，并鼓励开发其他项目，探索宿主-寄生虫相互作用、物种形成、行为和适应性特征获得的各个方面的遗传学。这种图谱提供的特定信息包括QTL分析、对单个QTL的遗传操作和解剖，以及识别单个QTL的潜在候选基因。

DESCRIPTION (provided by applicant): Peromyscines are the most widely distributed and abundant native North American mammals. Recently Peromyscus leucopus and P maniculatus abruptly rose to national attention when they were discovered to be the primary reservoirs of microbes causing two emerging infectious diseases: Lyme disease and Hantaviral pulmonary syndrome. Aptly called "The Drosophila of North American Mammology" peromyscines are also considered to be an ideal model for studying 1) the genes responsible for reproductive isolation and speciation, and 2) the genes enabling the physiological and behavioral adaptation to changing environmental conditions, adaptation to other species, adaptation to each other, and adaptation to microbial and other parasites. An important tool for exploiting this potential is a linkage map. It is the Aim of this proposal to develop an intermediate-density linkage map of P maniculatus bairdii using PCR based markers at sufficient density, approximately 5-10 cM, to permit identification of major segments syntenic with the reference species, Mus musculus. Such markers consist of 1) Type I (Protein Coding Genes) important for synteny identification, and 2) Type II (Microsatellites), which are highly polymorphic and will ultimately be used for QTL analysis. The linkage analysis panel was selected for maximizing utilizable polymorphism and is composed of Interspecific Meiotic Segregants of crosses between the sister species P m bairdii and P polionotus. Development of a Peromyscus linkage map will markedly improve the research potential of the species, enhancing projects currently underway and encouraging the development of other projects that explore the genetics of host-parasite interactions, speciation, behavior and the various aspects of adaptive trait acquisition. Specific information enabled by such a map includes QTL analysis, genetic manipulation and dissection of individual QTLs, and identification of potential candidate genes for individual QTLs.