PEROMYSCUS GENOMICS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Peromyscines are the most widely distributed and abundant native North American mammals. Recently Peromyscus leucopus and P maniculatus abruptly rose to national attention when they were discovered to be the primary reservoirs of microbes causing two emerging infectious diseases: Lyme disease and hantaviral pulmonary syndrome. Aptly called ¿The Drosophila of North American Mammology¿ peromyscines are also considered to be an ideal model for studying 1) the genes responsible for reproductive isolation and speciation, and 2) the genes enabling the physiological and behavioral adaptation to changing environmental conditions, adaptation to other species, adaptation to each other, and adaptation to microbial and other parasites. An important tool for exploiting this potential is a linkage map. It is the Aim of this proposal to develop an intermediate-density linkage map of P maniculatus bairdii using PCR based markers at sufficient density, ~5-10 cM, to permit identification of major segments syntenic with the reference species, Mus musculus. Such markers consist of 1) Type I (Protein Coding Genes) important for synteny identification, and 2) Type II (Microsatellites), which are highly polymorphic and will ultimately be used for QTL analysis. Linkage analysis panels were selected for maximizing utilizable polymorphism and include1) Interspecific Meiotic Segregants of crosses between the sister species P m bairdii and P polionotus , and 2)Whole Genome Radiation Hybrids in hamster cells. To these ends we are developing a collaborative multi-institutional group (Claflin, University of South Carolina (USC), and Savannah River Ecology Laboratory (SREL)) to develop genomic resources that will train students and faculty in biomedical research methods, enhance the research capabilities of our institutions,