PEROMYSCUS GENOMICS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。Peromyscines是分布最广泛和最丰富的北美本土哺乳动物。最近，白足鹿鼠（Peromyscus leucopus）和马尼拉鹿鼠（P maniculatus）突然引起了全国的关注，因为它们被发现是引起两种新出现的传染病（莱姆病和汉坦病毒肺综合征）的主要微生物宿主。 恰当地称为北美哺乳动物的果蝇也被认为是研究1）负责生殖隔离和物种形成的基因，以及2）使生理和行为适应不断变化的环境条件，适应其他物种，相互适应以及适应微生物和其他寄生虫的基因的理想模型。 利用这种潜力的一个重要工具是连锁图。这是本建议的目的是开发一个中等密度的连锁图谱的P maniculatus bairdii使用PCR标记在足够的密度，约5-10 cM，允许识别的主要部分同线的参考物种，小家鼠。这些标记包括1）对同线性鉴定重要的I型（蛋白质编码基因），和2）高度多态性并最终将用于QTL分析的II型（微卫星）。选择连锁分析组以使可利用的多态性最大化，包括1）姐妹种P m bairdii和P polionotus之间杂交的种间减数分裂分离体，和2）仓鼠细胞中的全基因组辐射杂交体。 为此，我们正在建立一个多机构协作小组（克拉夫林、南卡罗来纳州大学（USC）和萨凡纳河生态实验室（SREL）），以开发基因组资源，培训学生和教师掌握生物医学研究方法，提高我们机构的研究能力，

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Peromyscines are the most widely distributed and abundant native North American mammals. Recently Peromyscus leucopus and P maniculatus abruptly rose to national attention when they were discovered to be the primary reservoirs of microbes causing two emerging infectious diseases: Lyme disease and hantaviral pulmonary syndrome. Aptly called ¿The Drosophila of North American Mammology¿ peromyscines are also considered to be an ideal model for studying 1) the genes responsible for reproductive isolation and speciation, and 2) the genes enabling the physiological and behavioral adaptation to changing environmental conditions, adaptation to other species, adaptation to each other, and adaptation to microbial and other parasites. An important tool for exploiting this potential is a linkage map. It is the Aim of this proposal to develop an intermediate-density linkage map of P maniculatus bairdii using PCR based markers at sufficient density, ~5-10 cM, to permit identification of major segments syntenic with the reference species, Mus musculus. Such markers consist of 1) Type I (Protein Coding Genes) important for synteny identification, and 2) Type II (Microsatellites), which are highly polymorphic and will ultimately be used for QTL analysis. Linkage analysis panels were selected for maximizing utilizable polymorphism and include1) Interspecific Meiotic Segregants of crosses between the sister species P m bairdii and P polionotus , and 2)Whole Genome Radiation Hybrids in hamster cells. To these ends we are developing a collaborative multi-institutional group (Claflin, University of South Carolina (USC), and Savannah River Ecology Laboratory (SREL)) to develop genomic resources that will train students and faculty in biomedical research methods, enhance the research capabilities of our institutions,