PEROMYSCUS GENOMICS

白鼠基因组学

• 批准号: 7391983
• 负责人: MICHAEL J DEWEY
• 金额: $ 6.72万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391983
• 关键词: Drosophilidae; Lyme disease; Mus musculus; attention; conditioning; density; ecology; genes; genome; hamsters; linkage mapping; model; proteins; siblings; syndrome; training; university

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Peromyscines are the most widely distributed and abundant native North American mammals. Recently Peromyscus leucopus and P maniculatus abruptly rose to national attention when they were discovered to be the primary reservoirs of microbes causing two emerging infectious diseases: Lyme disease and hantaviral pulmonary syndrome. Aptly called ¿The Drosophila of North American Mammology¿ peromyscines are also considered to be an ideal model for studying 1) the genes responsible for reproductive isolation and speciation, and 2) the genes enabling the physiological and behavioral adaptation to changing environmental conditions, adaptation to other species, adaptation to each other, and adaptation to microbial and other parasites. An important tool for exploiting this potential is a linkage map. It is the Aim of this proposal to develop an intermediate-density linkage map of P maniculatus bairdii using PCR based markers at sufficient density, ~5-10 cM, to permit identification of major segments syntenic with the reference species, Mus musculus. Such markers consist of 1) Type I (Protein Coding Genes) important for synteny identification, and 2) Type II (Microsatellites), which are highly polymorphic and will ultimately be used for QTL analysis. Linkage analysis panels were selected for maximizing utilizable polymorphism and include1) Interspecific Meiotic Segregants of crosses between the sister species P m bairdii and P polionotus , and 2)Whole Genome Radiation Hybrids in hamster cells. To these ends we are developing a collaborative multi-institutional group (Claflin, University of South Carolina (USC), and Savannah River Ecology Laboratory (SREL)) to develop genomic resources that will train students and faculty in biomedical research methods, enhance the research capabilities of our institutions,

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。 Peromyscines 是分布最广、数量最多的北美本土哺乳动物。最近，白足鼠（Peromyscus leucopus）和足足鼠（Pomyscus maniculatus）被发现是引起两种新发传染病（莱姆病和汉坦病毒肺综合征）的微生物的主要宿主，从而突然引起全国关注。 被恰当地称为“北美哺乳动物学果蝇”的peromyscines也被认为是研究1）负责生殖隔离和物种形成的基因，2）使生理和行为适应不断变化的环境条件、适应其他物种、相互适应以及适应微生物和其他寄生虫的基因的理想模型。 开发这种潜力的一个重要工具是链接图。本提案的目的是使用基于 PCR 的标记以足够的密度（~5-10 cM）开发 P maniculatus bairdii 的中等密度连锁图谱，以允许鉴定与参考物种 Mus musculus 同线的主要片段。此类标记包括 1) 对于同线性鉴定很重要的 I 型（蛋白质编码基因）和 2) II 型（微卫星），它们具有高度多态性，最终将用于 QTL 分析。选择连锁分析组以最大化可利用的多态性，包括 1) 姐妹种 P m bairdii 和 P poliionotus 之间杂交的种间减数分裂分离，以及 2) 仓鼠细胞中的全基因组辐射杂交。 为此，我们正在建立一个多机构协作小组（克拉夫林、南卡罗来纳大学 (USC) 和萨凡纳河生态实验室 (SREL)）来开发基因组资源，培训学生和教师生物医学研究方法，增强我们机构的研究能力，