Structural and functional studies of ion transporters.

描述（由申请人提供）：离子同调体是介导两种不同离子在膜上的共同运输的膜蛋白。它们在人体生理学中起着重要作用；例如，Na+/i-同调体负责甲状腺中i-的积累。尽管它们很重要，但缺乏对symports如何工作的详细的机制理解。这项提议的长期目标是发展一个详细的分子模型，为两种离子，Na+和K+的共同运输，由细菌同质转运体KtrAB。

DESCRIPTION (provided by applicant): Ion symporters are membrane proteins that mediate the co-transport of two different ion species across themembrane. They play important roles in human physiology; the Na+/i- symporter, for example, is responsible for accumulation of I- in the thyroid gland. Despite their importance a detailed mechanistic understanding of how symporters work is lacking. The long term objective of this proposal is the development of a detailed molecular model for the co-transport oftwo ions, Na+ and K+ by KtrAB, a bacterial symporter. The major aims of this proposal are: 1 - Characterize the transport properties of the KtrAB symporter. 2 - Characterize the structure and function of KtrA. 3 - Determine the crystal structure of the membrane protein KtrB.