Acquisition of a LC-MS/MS Proteomics System

描述（由申请人提供）：申请资金购买商业蛋白质组学分析LC-MS/MS系统，专用于生物实验，其中蛋白质鉴定，蛋白质定量和蛋白质组谱的时间分辨分析以及翻译后修饰目前正在进行中。新的LC-MS/MS系统将被安置在康涅狄格大学健康中心的蛋白质组学和生物质谱设施（PBMS设施）。该研究中心为UCHC的研究社区提供高灵敏度的微毛细管液相色谱-串联质谱仪器和蛋白质组学相关资源。预计使用PBMS设施的20多个实验室和30多名研究生和博士后学生中的许多人将利用灵敏的LCMS/ MS系统的能力来推进广泛的研究计划。这里介绍了来自5个主要用户的项目，这些用户在UCHC有完善的项目。所有这些都需要Finnigan LCQ-XP离子阱技术提供的灵敏和快速的蛋白质鉴定和同时定量，并通过使用独特的SEQUEST、XPRESS、INTERACT和Proteome 3D软件工具进行并行计算机数据库分析。大卫韩博士将利用3D-ICAT技术，通过差异ICAT标记来鉴定和定量凋亡细胞膜上呈现的凋亡信号蛋白和吞噬配体。Timothy Hla博士将使用小鼠模型分析EDG 1受体缺失或考克斯-2酶过表达直接导致的蛋白质组学变化。Dan Wu博士将分析Wnt蛋白和化学引诱物信号转导诱导的膜和细胞溶质蛋白质组学变化。Glen King结构生物学的同事将利用质谱来描述多个蛋白质复合物的结构-功能研究。布鲁斯迈耶博士将利用质谱法鉴定SH 2相互作用蛋白。1999年开设了一座新的学术研究大楼，在UCHC招募了40多名新的教职员工，从1996年到2002年增加了4000多万美元的资金，最近获得了利用蛋白质组学技术的计划项目赠款申请，和重要的出版物利用蛋白质组学技术证明了需要一个额外的新的蛋白质组学工作站在大学康涅狄格州健康中心

DESCRIPTION (provided by applicant): Funds are requested to purchase a commercial Proteomics analysis LC-MS/MS system dedicated for biological experiments where protein identification, protein quantification, and time-resolved analysis of proteome profiling, and post-translation modification are currently in progress. The new LC-MS/MS system will be housed at the Proteomics and Biological Mass Spectrometry Facility (PBMS facility) at the University of Connecticut Health Center. This research center offers use of highly sensitive micro-capillary liquid chromatography-coupled to Tandem Mass Spectrometry instrumentation and proteomics related resources to the research community at the UCHC. It is anticipated that many of the over 20 laboratories and over 30 graduate and postdoctoral students using the PBMS facility will employ capabilities of the sensitive LCMS/ MS system to advance the wide range of research programs. Projects from 5 major users with well developed projects at the UCHC are described here. All of these requires the sensitive and rapid protein identification and simultaneous quantification afforded by the Finnigan LCQ-XP ion trap technology coupled to the parallel-computer database analysis by the use of uniquely available SEQUEST, XPRESS, INTERACT, and Proteome 3D software tools. Dr. David Han will utilize 3D-ICAT technology to identify and quantify apoptotic signaling proteins and engulfment ligands that are presented on apoptotic cell membranes by differential ICAT labeling. Dr. Timothy Hla will analyze proteomics changes that are direct result of the EDG1 - receptor deletion or COX-2 enzyme overexpression using mouse models. Dr. Dan Wu will analyze membrane and cytosolic proteomics changes that are induced by Wnt protein and chemo-attractant signal transduction. Dr. Glen King structure biology colleagues will utilize mass spectrometry to delineate structure-function studies of multiple protein complexes. Dr. Bruce Mayer will utilize mass spectrometry to identify SH2 interacting proteins. Opening of a new academic research building in 1999 and recruitment of over 40 new faculty members at the UCHC, the increase in funding of over 40 million from 1996-2002, the recent award of a Program Project grant application that utilize proteomics technologies, and significant publications utilization proteomics technologies attest to the need for an additional new Proteomics Workstation at the University of Connecticut Health Center.