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Structural Biology of sulfotransferase and glycosyltrans

Structural Biology of sulfotransferase and glycosyltrans
磺基转移酶和糖基反式的结构生物学
批准号：
7007467
负责人：
MASAHIKO NEGISHI
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7007467
关键词：
X ray crystallographyenvironmental toxicologyenzyme activityenzyme structureenzyme substrate complexglucuronosyltransferaseglycosyltransferasehalobiphenyl /halotriphenyl compoundhemagglutininheparan sulfatemicrocalorimetrymucopolysaccharidesprotein protein interactionsite directed mutagenesisstructural biologysulfationsulfotransferase

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中文摘要
翻译
细胞溶质ST硫酸化异种化学物质用于解毒和分泌，并且还硫酸化生物信号分子如类固醇激素和生物胺用于灭活和/或储存。某些硫酸盐产品会致癌。高尔基体膜ST对多糖的硫酸化位点具有高度特异性。各种糖基转移酶合成多糖并为ST提供底物。我们的研究目标是阐明这些酶的结构和功能。为此，我们利用细菌表达系统生产了大量的重组蛋白，并将其结晶，并解决了它们的三维结构。
英文摘要
Cytosolic STs sulfate xenochemicals for detoxification and secretion and also sulfate biological signal molecules such as steroid hormones and bioamines for inactivation and/or storage. Certain sulfated products become carcinogenic. Golgi-membrane STs display high specificity to sulfate distinct sites of polysaccharides. Various glycosyltransferases synthesize polysaccharides and provide STs with substrates. Our research objectives are to eluciadte structure and function of these enzymes. For this, we have used bacterial expression system to produce a large amount of recombinant proteins, have crystallized them, and have solved their 3-dimensional structures.
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