Applications of Time-Reverse Tissue Turbidity Suppression to Improve Biophotonics

描述（由申请人提供） 翻译后摘要：生物组织散射光非常强烈。这阻止了光通过组织的有效传输，并严重限制了基于光学的生物医学传感和成像技术从深层组织区域提取有用信息的能力。我们建议应用独特的方面，新发现的生物光学现象，称为组织浊度抑制通过光学相位共轭（TSOPC），诱导有限形式的组织透明度通过抑制光散射，并开发新的和改进类的光学显微镜，生物传感和光学治疗技术。组织散射可能看起来是随机的，但它们基本上是确定性的和时间可逆的。具体地说，如果我们能够记录并再现通过组织的漫射光透射的时间反转副本，我们实际上可以迫使光场重新追踪其通过组织的路径并恢复原始输入光场。我们最近发现了这一现象，并发现它在抑制活组织中的散射光方面具有惊人的鲁棒性和有用性。这种处理组织散射的新方法是非常不寻常的，因为它代表了与当前光学技术处理组织散射的方式的巨大偏离。该申请提出应用这种现象的独特性质来增加通过组织的光传输，在组织深处产生紧密聚焦的光斑-现有技术无法实现的能力。这些能力可以显著地扩展光学状态感测和成像技术的应用范围。我们建议调整这些功能，以创建高度敏感的非侵入性生化传感器，最终可能允许血液和组织分析而无需抽血，改进高分辨率3D显微镜，开发独特的深层组织生化成像系统，并实现灵活的手段，将光深入和精确地传递到组织中，用于光动力学治疗应用。 公共卫生相关性：这项工作旨在进一步研究一种新发现的生物光学现象，这种现象可以通过抑制光学散射来诱导有限形式的组织透明度。我们计划适应这种现象的独特功能：1）建立免抽血的高灵敏度血液和组织分析（如葡萄糖测量）系统，2）改进显微镜，3）实施深层组织生化成像系统，以及4）通过光动力疗法有效地将光传递到组织深处，以实现微创癌症肿瘤切除。

DESCRIPTION (Provided by the applicant) Abstract: Biological tissues scatter light very strongly. This prevents efficient light transmission through tissues and severely limits the abilities of optical-based biomedical sensing and imaging technologies to extract useful information from deep tissue regions. We propose to apply unique aspects of a newly discovered bio-optical phenomenon, termed tissue turbidity suppression by optical phase conjugation (TSOPC), to induce a limited form of tissue transparency by suppressing optical scattering, and to develop new and improved classes of optical microscopy, biosensing and optical therapeutic techniques. Tissue scattering may appear random but they are fundamentally deterministic and time- reversible. Specifically, if we can record and reproduce a time-reversed copy of the diffuse light transmission through a tissue, we can actually force the light field to retrace its paths through the tissue and recover the original input light field. We recently uncovered this phenomenon and found that it is surprisingly robust and useful in suppressing scattering light in living tissues. This new way of tackling tissue scattering is highly unusual as it represents a big departure from the ways current optical techniques address tissue scatterings. This application proposes to apply the unique properties of this phenomenon to increase light transmission through tissues, generate tightly focused light spots deep within tissues - capabilities that are unattainable by existing technologies. These capabilities can significantly expand the application range of optical regime sensing and imaging techniques. We propose to adapt these capabilities to create highly sensitive non-invasive biochemical sensors that may eventually allow blood-and-tissue analysis without blood draw, improve high-resolution 3D microscopes, develop a unique deep tissue biochemical imaging system, and implement a flexible means to deliver light deeply and precisely into tissues for photodynamic therapy applications. Public Health Relevance: The proposed work aims to further the research on a newly uncovered bio-optical phenomenon that can induce a limited form of tissue transparency by suppressing optical scattering. We plan to adapt unique features of this phenomenon 1) to build blood-draw-free highly sensitive blood-and-tissue analysis (such as glucose measurement) systems, 2) improve microscopy, 3) to implement a deep tissue biochemical imaging system, and 4) to efficiently deliver light deep into tissues for minimally invasive cancer tumor removal via photodynamic therapy.