Core-C-chronic hypoxia model

该低氧-高碳酸血症核心将以各种方式服务于该计划中的4个项目。核心C将以标准化和一致的方式提供暴露于缺氧或高碳酸血症（或两者）的小鼠（或大鼠）。此外，该核心将促进调查人员之间的互动，并有助于开辟合作调查的新途径，因为它使用相同的模式来研究应对和适应长期和 梯度应力 该核心基金的目标如下： 1.确保使用一致的慢性缺氧或高碳酸血症或两者的实验模型 在所有四个项目中; 2.培养技术人员和博士后，帮助所有人进行组织解剖和收集 研究人员，无论是大脑、心脏还是肾脏; 3.继续表征慢性低氧高碳酸血症模型，以便更好地 了解在这个程序的过程中出现的新发现的含义; 4.建立具有遗传性的小鼠慢性缺氧或高碳酸血症的可比模型， 改变（例如敲除，如NHE-/-），以提出特定的适应机制，或 伤害; 5.为了帮助研究人员设计需要长期暴露于细胞中的实验， 在低氧、高碳酸血症或两者中培养延长的时间。

This Hypoxia-Hypercapnia Core will serve the 4 Projects in the Program in various ways. Core C will provide mice (or rats) that have been exposed to hypoxia or hypercapnia (or both) in a standardized and consistent way. Moreover, the Core will promote interactions among the investigators and help generate new avenues of collaborative investigation because of the use of identical models to study mechanisms for response and adaptation to long-term and graded stress. The aims of this Core Facility are as follows: 1. To ensure a consistent experimental model of chronic hypoxia or hypercapnia or both for use in all four projects; 2. To train technicians and postdoctoral and help in tissue dissection and collection for all investigators whether brain, heart or kidney; 3. To continue to characterize the chronic hypoxia-hypercapnia model in order to better understand the implications of novel findings that arise during the course of this program; 4. To develop comparable models of chronic hypoxia or hypercapnia in mice with genetic alterations (e.g. Knock-out such as NHE-/-) to propose specific mechanisms for adaptation or injury; 5. To help investigators in the design of experiments requiring chronic exposure of cells in culture for an extended period of time in hypoxia, hypercapnia or both.