Novel 3D printing approaches to induce intestinal epithelium development from adult derived gut organoids

该项目将开发一个正常肠上皮的体外3D模型，以实现一个基于细胞的模型，更紧密地复制体内所见的肠上皮结构和功能。这样的模型将解决与有机培养相关的巨大变异性，这导致了两次实验之间的重复性问题。我们使用3D打印来产生3D结构的创新方法，模拟了体内看到的几何和细胞组织，将在体外准确地复制肠道上皮，并最终支持基础生物科学的翻译。因此，这一跨学科的研究项目提供了一个令人兴奋的机会，使用最先进的生物打印技术重新定义上皮组织的3D培养。学生将使用添加剂生物制造，包括添加剂制造和微流体，允许在动态培养条件下形成具有特定结构的3D细胞培养基质。此外，成功的应聘者将能够发展哺乳动物细胞培养的技能，包括原代细胞的培养，以及培养后的特征。

This project will develop an in vitro 3D model of the normal, intestinal epithelium to achieve a cell-based model that more closely replicates intestinal epithelial structure and function as seen in vivo. Such a model will address the enormous variability associated with organoid culture which leads to issues regarding reproducibility between experiments. Our innovative approach of using 3D printing to produce 3D structures that mimic the geometry and cellular organisation as seen in vivo, will accurately reproduce the intestinal epithelium in vitro and ultimately support the translation of basic bioscience.This inter-disciplinary research project therefore provides an exciting opportunity to redefine 3D culture of epithelial tissues using state-of-the-art bioprinting techniques. The student will utilise additive biofabrication, including additive manufacturing and microfluidics, that allow the formation of 3D cell culture substrates with defined architectures within dynamic culture conditions. In addition, the successful candidate will be able to develop skills in mammalian cell culture, including of primary cells, and in their characterisation following culture.