Characterization of candidate histone methyltransferases

我们对两个候选组蛋白甲基转移酶在人类和小鼠组织中的转录进行了全面的分析，显示了选择性剪接和通读转录产物的组织特异性表达。我们进行了几次酵母双杂交筛选，以确定可能的组蛋白甲基转移酶的蛋白结合伙伴。这些筛选产生了几个耐人寻味的候选者，并通过生化和遗传方法进一步确定了这些候选者的特征。我们已经构建了一个条件性基因敲除质粒，以产生一个基因敲除的小鼠模型，该模型缺乏一个位于经常缺失的染色体区域的假定的组蛋白甲基转移酶。

We conducted a comprehensive analysis of transcription of two candidate histone methyltransferases in human and mouse tissues, showing tissue-specific expression of alternatively spliced and read-through transcripts. We conducted several yeast two-hybrid screens to identify protein-binding partners for the putative histone methyltransferases. These screens yielded several intriguing candidates that are being characterized further by biochemical and genetic methods. We have constructed a conditional knockout plasmid to generate a knockout mouse model lacking one of the putative histone methyltransferases located on a frequently deleted chromosomal region.