Rapid detection of food borne pathogenic bacteria

描述（由申请人提供）：本提案的目的是开发一种超声颗粒凝集（UPA）方法，用于测定体液、食品和环境样本中的致病菌大肠杆菌O157：H7和单核细胞增生李斯特菌。UPA检测基于超声的使用，以显著提高灵敏度并减少常规颗粒凝集免疫测定的时间。超声波用于加速和监测凝集过程。 细菌的有效检测需要满足许多具有挑战性的标准的分析方法。细菌检测方法必须是快速和非常敏感的，因为即使在身体或食物中存在单一的病原体也可能是感染剂量。我们建议开发的免疫测定将在一个简单，便携和廉价的设备的基础上，新颖的专利圆柱形谐振器技术。值得注意的是，该方法可以很容易地适用于检测其他目标对象。初步研究证实了拟议技术的主要创新组成部分的可行性，并表明新方法具有重大的商业和科学潜力。

DESCRIPTION (provided by applicant): The objective of this proposal is to develop an ultrasound particle agglutination (UPA) method for determination of pathogenic bacteria Escherichia coli O157:H7 and Listeria monocytogenes in body fluids, food and environmental samples. The UPA assay is based on the use of ultrasound to significantly increase the sensitivity and reduce time of conventional particle agglutination immunoassay. Ultrasound waves are used for both accelerating and monitoring of the agglutination process. The effective testing of bacteria requires methods of analysis that meet a number of challenging criteria. Bacterial detection methods have to be rapid and very sensitive since the presence of even a single pathogenic organism in the body or food may be an infectious dose. The immunoassay we propose to develop will be implemented in a simple, portable and inexpensive device based on the novel patented cylindrical resonator technology. Significantly, the method can be easily adapted to detect other target objects. Preliminary studies confirmed feasibility of the major innovative components of the proposed technology and it is shown that the new method has significant commercial and scientific potential.