Core--ANIMAL, GENOTYPING, CELL & TISSUE (AGCT)

动物、基因分型、细胞和组织(AGCT)核心将为饲养动物提供技术和人员支持，促进、标准化和实施动物基因分型以及组织样本的收集、管理和分析。AGCT核心设施将在以下方面为需要援助的所有研究人员提供服务：(1)动物护理、繁殖和免疫，(2)组织和/或体液的收集，(3)标记辅助回交(“快速同源”)过程中NY Finenian、Zhang和Mikecz项目的所有基因分型，(4)基本细胞培养方法，(5)用于评估关节炎发展和进展的标准化测试，以及(6)组织学和评估组织样本的处理样本。AGCT核心计划通过提供技术、仪器和人员支持来帮助每个项目，这些支持是(I)几个项目常用的，或(Ii)调查人员偶尔使用的，但需要特别的技术或安全条例，或(Iii)特殊设备或技术经验。此外，AGCT核心还提供基本的统计和必要的生物信息学分析。根据这一应用的预期需求，AGCT核心设施将由四个单元组成。动物护理核心单位将设计和执行基因控制的饲养，根据研究人员的需要为动物接种疫苗，并以独立于PI(盲目)的方式评估关节炎的进展。因此，该股将为所有生产和测试供个别项目使用的动物的项目提供服务。基因分型核心单位为回交过程中所有需要基因分型的项目提供服务。这包括为基因组DNA分离、记录保存和标记动物收集样本，以及对动物进行基因分型。 菌株特异性多态标记。由于动物数量多，多个同源/亚同源品系，格兰特项目的标记辅助育种过程和选择具有适当(所需)重组的动物不属于这一核心。另一方面，同一人(该核心的负责人和格兰特项目的联合调查员)将控制所有动物育种和基因分型实验。细胞和组织测试核心单位将通过收集动物组织和人类软骨、准备免疫抗原以及用于标准化测试的组织和血清样本来支持所有调查人员。该股将负责样本储存、记录保存和收集样本的数据管理，并对组织标本进行评估/评分。这个核心单位将帮助研究人员收集细胞或组织进行专门分析(例如，外周血、脾细胞或建立原代细胞培养)，执行细胞的组织学和/或基因分型测试，并存储组织样本以供进一步分析。生物统计生物信息学核心股将标准化和进行统计分析，并在数据管理方面为调查人员提供支持。

The Animal, Genotyping, Cell & Tissue (AGCT) Core will provide technical and personnel support for breeding animals, facilitating, standardizing, and implementing animal genotyping and the collection, management and analysis of tissue samples. The AGCT Core Facility will serve all investigators who will be needing assistance with (1) animal care, breeding and immunization, (2) collection of tissue and/or body fluid, (3) all genotyping for Projects ny Finnegan, Zhang, and Mikecz along the marker-assisted backcrossing ("speed congenic") processes, (4) basic cell culture methods, (5) standardized testing for assessment of the development and progression of arthritis, and (6) processing samples for histology and evaluation of tissue samples. The AGCT Core intends to aid each project by providing technical, instrumental and personnel support which are (i) commonly used by several projects or (ii) occasionally used by investigators but require special technical or safety regulations or (iii) special equipment or technical experience. In addition, the AGCT Core offers basic statistical and necessary bioinformatic analysis. The AGCT Core facility, based on the expected demands of this application, will consist of four units. The Animal Care Core Unit will design and perform genotype-controlled breeding, immunize animals according to the needs of the investigators and assess the progression of arthritis in PI-independent (blind) manner. Thus, this Unit will serve all projects generating and testing animals ready for use by individual projects. The Genotyping Core Unit serves all projects needing genotyping along the backcrossing processes. This includes sample collection for genomic DNA isolation, record keeping and labeling animals, and genotyping of animals with strain-specific polymorphic markers. Due to the large number of animals and multiple congenic/sub-congenic lines, the marker-assisted breeding process of Project by Glant and selection of animals with appropriate (required) recombinations does not belong to this core. On the other hand, the same person (director of this core and co-investigator of Project by Glant ) will control all animal breeding and genotyping experiments. The Cell and Tissue Testing Core Unit will support all investigators by collecting animal tissues and human cartilage, preparing antigens for immunization, and tissue and serum samples for standardized testing. This unit will be responsible for sample storage, record keeping and data management of collected samples, and evaluate/score histological specimens. This core unit will help investigators collect cells or tissues for specialized analysis (e.g. peripheral blood, spleen cells, or establish primary cell cultures), perform histology and/or genotyping tests of cells, and store tissue samples for further analyses. The BiostatisticallBioinformatics Core Unit will standardize and perform statistical analysis and support investigators in data management.