CLINICAL IMMUNOLOGY LABORATORY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。遗传性免疫缺陷疾病是一类重要的遗传疾病。Peter Felsburg博士在宾夕法尼亚大学兽医学院为狗和猫建立了一个全方位服务的临床免疫学实验室，这将有助于筛选实验室识别患有原发性免疫缺陷疾病的伴侣动物。 实验室提供的检测包括流式细胞术，用于测定犬中的：CD 3、CD 4、CD 8、CD 14、CD 21、CD 45 RA、TCR α和TCR gd;猫中的：CD 5、CD 4、CD 8、CD 14和CD 21。此外，用于检测缺陷性白细胞整联蛋白表达的组包括CD 11 a、CD 11b、CD 11 c和CD 18。我们现在还提供血清免疫球蛋白定量（IgG，IgM和伊加），淋巴细胞增殖试验和中性粒细胞/单核细胞功能测试，测量过氧化氢的产生。通过这一系列测试，我们将能够更好地识别免疫功能中的遗传异常，扩大发现人类遗传疾病同源物的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Inherited immunodeficiency disorders are an important class of genetic disease. Dr. Peter Felsburg has instituted a full-service clinical immunology laboratory for dogs and cats at the Veterinary School, University of Pennsylvania, which will aid the screening laboratory in identifying companion animals with primary immunodeficiency diseases. The testing provided by the laboratory includes flow cytometry to determine in dogs: CD3, CD4, CD8, CD14, CD21, CD45RA, TCRa¿, and TCRgd; in cats: CD5, CD4, CD8, CD14, and CD21. In addition, a panel to detect defective leukocyte integrin expression includes CD11a, CD11b, CD11c, and CD18. We also now offer serum immunoglobulin quantitation (IgG, IgM, and IgA), a lymphocyte proliferation assay, and a neutrophil/monocyte function test that measures hydrogen peroxide production. With this battery of tests, we will be better able to identify genetic abnormalities in immune function, expanding the capacity to uncover homologes of human genetic disease.