PEROMYSCUS GENOMICS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。长孢菌科动物是分布最广、数量最多的北美本土哺乳动物。近年来，白斑拟青霉和黑线拟青霉被发现是引起莱姆病和汉坦病毒肺综合征两种新发传染病的主要微生物储存库，突然引起了全国的关注。被恰当地称为北美哺乳动物学中的果蝇也被认为是研究1)负责生殖隔离和物种形成的基因，以及2)使生理和行为适应变化的环境条件，适应其他物种，相互适应，以及适应微生物和其他寄生虫的理想模型。开发这一潜力的一个重要工具是连锁图谱。本研究的目的是在足够的密度(~5-10 cM)下，利用基于聚合酶链式反应的标记，建立一张贝氏小蜂的中等密度连锁图谱，以便于鉴定与参考种小鼠共线的主要片段。这些标记包括1)类型I(蛋白质编码基因)和2)类型II(微卫星)，类型I(蛋白质编码基因)对共性鉴定很重要，类型II(微卫星)具有高度多态，最终将用于QTL分析。选择连锁分析小组是为了最大限度地利用多态，包括1)姊妹种P m bairdii与小儿麻痹症患者杂交的种间减数分裂片段，以及2)仓鼠细胞中的全基因组辐射杂交。为此，我们正在发展一个协作的多机构小组(克拉夫林，南卡罗来纳大学(USC)和萨凡纳河生态实验室(SREL))，以开发基因组资源，将培训学生和教师的生物医学研究方法，增强我们机构的研究能力，

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Peromyscines are the most widely distributed and abundant native North American mammals. Recently Peromyscus leucopus and P maniculatus abruptly rose to national attention when they were discovered to be the primary reservoirs of microbes causing two emerging infectious diseases: Lyme disease and hantaviral pulmonary syndrome. Aptly called ¿The Drosophila of North American Mammology¿ peromyscines are also considered to be an ideal model for studying 1) the genes responsible for reproductive isolation and speciation, and 2) the genes enabling the physiological and behavioral adaptation to changing environmental conditions, adaptation to other species, adaptation to each other, and adaptation to microbial and other parasites. An important tool for exploiting this potential is a linkage map. It is the Aim of this proposal to develop an intermediate-density linkage map of P maniculatus bairdii using PCR based markers at sufficient density, ~5-10 cM, to permit identification of major segments syntenic with the reference species, Mus musculus. Such markers consist of 1) Type I (Protein Coding Genes) important for synteny identification, and 2) Type II (Microsatellites), which are highly polymorphic and will ultimately be used for QTL analysis. Linkage analysis panels were selected for maximizing utilizable polymorphism and include1) Interspecific Meiotic Segregants of crosses between the sister species P m bairdii and P polionotus , and 2)Whole Genome Radiation Hybrids in hamster cells. To these ends we are developing a collaborative multi-institutional group (Claflin, University of South Carolina (USC), and Savannah River Ecology Laboratory (SREL)) to develop genomic resources that will train students and faculty in biomedical research methods, enhance the research capabilities of our institutions,