Establishment of a UMDNJ NeuroProteomics Core Facility

描述(由申请人提供)： 蛋白质组学的发展为神经系统疾病相关研究提供了前所未有的机遇。在新泽西医学与牙科大学(UMDNJ)，我们组建了一个由七名NINDS研究人员组成的团队，他们的研究和合作-包括探索神经元分化、突触稳定性和炎症，以及生物钟、帕金森氏病和巴顿病的生物机制-将因蛋白质组学核心的推出而显著增强。因此，我们建议使用NINDS中心核心赠款在UMDNJ建立神经蛋白质组学核心设施，这一资源不会与现有设施重叠。蛋白质组学的成功应用需要最先进的仪器和经验丰富的操作员，UMDNJ已经采取措施将两者组装起来。利用新泽西州高等教育委员会的资金，以及美国国立卫生研究院和美国国家科学基金会的额外资金支持，CORE的大部分设备已经购买并安装在新装修的1,000平方英尺的高级蛋白质组学研究中心，指导人员是具有神经科学背景的经验丰富的质谱学家弘力博士。此外，UMDNJ为空间翻新拨出了大量财政支助，并支付了首席调查员的大部分工资。NINDS核心赠款将用于支付CAPR业务成本的三分之一，方法是提供样品制备、二维凝胶电泳、质谱学和生物信息学方面的专职人员，以及与NINDS资格项目相关的服务合同和用品。我们迅速发展的当地神经科学研究社区(包括UMDNJ的30多名NINDS调查人员)也将可以使用这一设施。由NINDS和其他CAPR调查人员组成的两个委员会将监督该设施的财政和科学管理以及日常运营。用户俱乐部将为数据共享和协作提供一个重要的论坛。在NINDS拨款的帮助下，UMDNJ的神经科学研究人员可以最大限度地利用最先进的蛋白质组学技术：补充他们现有的研究方法，促进新的研究方向和合作。

DESCRIPTION (provided by applicant): Advances in proteomics have provided unprecedented opportunities for neurological disease related research. At the University of Medicine and Dentistry of New Jersey (UMDNJ), we have assembled a team of seven NINDS investigators whose research and collaborations - work that includes the exploration of neuronal differentiation, synapse stability, and inflammation, as well as the biological mechanisms of circadian clock, Parkinson's disease, and Batten disease - would be significantly enhanced by the availability of a proteomics Core. Thus, we propose using a NINDS center core grant to establish a NeuroProteomics Core Facility at UMDNJ, a resource that would not overlap with existing facilities. Successful application of proteomics requires both state-of-the-art instrumentation and experienced operators, and UMDNJ has already taken steps to assemble both. Using funds from the New Jersey Commission on Higher Education, with additional financial support from the NIH and NSF, most of the Core's equipment has already been purchased and installed in a newly renovated 1000-square-foot Center for Advanced Proteomics Research (CAPR) under the direction of Dr. Hong Li, an experienced mass spectrometric with a neuroscience background. Moreover, UMDNJ has allocated significant financial support for space renovation, as well as the bulk of the principle investigator's salary. An NINDS core grant would be used to cover one-third of the CAPR operational costs by providing dedicated personnel in sample preparation, 2D gel electrophoresis, mass spectrometry and bioinformatics, as well as service contracts and supplies associated with NINDS qualifying projects. Our rapidly growing local neuroscience research community (including over 30 NINDS investigators at UMDNJ) would also have access to this facility. Two committees, composed of both NINDS and other CAPR investigators, would oversee the facility's fiscal and scientific management as well as its daily operation. A User Club would provide an important forum for data sharing and collaborations. With the benefit of a NINDS grant, neuroscience investigators at UMDNJ could use state of-the art proteomics technology to maximum benefit: to complement their existing research approaches and promote new research directions and collaborations.