PROTEIN & NUCLEIC ACID CORE FACILITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。UCC医学院进行的研究涉及不同领域，包括行为神经药理学、修复机制、DNA损伤动力学、神经元和肌肉受体研究、细胞生理学以及毒素及其受体的分析。CMBC的蛋白质和核酸核心设施（PNACF）喜欢帮助从更经典的研究过渡到更分子和更详细的问题分析，由其教职员工解决。PNACF将专注于教师培训、研讨会、专业分子生物学技术、蛋白质表达、蛋白质纯化和表征方面的培训。因此，PNACF仍然是该机构研究维护和未来发展的重要核心设施。改善该设施的具体目标是：1。继续获得以更详细和现代的方式分析基本生物医学问题所需的分子工具。(2)赞助？邀请该领域专家举办分子生物学技术、蛋白质表达和蛋白质纯化技术研讨会。这将有助于UCC的研究人员解决他们的问题，并获得他们研究领域的初步数据。(3)在UCC建立蛋白质表达和纯化设施，鼓励UCC教师将研究扩展到蛋白质水平。(4)继续和扩大资源中心，使UCC的教员可以获得UCC所没有的分子生物学技术和服务的技术信息和成本估算。(5)继续和改进核酸和蛋白质数据的计算机化分析；(6)实施？服务费用？该计划将用于Bio-RAD的VersaDOC系统的服务合同/维修。这是为了让RCMI项目的PNACF部分得到部分的自我支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The research performed at the UCC medical school are in different fields from behavioral neuropharmacology, repair mechanism, kinetics of DNA damage, neuronal and muscular receptor studies, cellular physiology and the analysis of toxin with its receptor. The Protein and Nucleic Acid Core Facility (PNACF) of the CMBC likes to aid in the transition from the more classical studies to a more molecular and detailed analysis of the problems being addressed by its faculty members. PNACF will focus on faculty training, seminars, training on specialized molecular biology techniques, protein expression and protein purification and characterization. Therefore, the PNACF continues to be an important core facility for the maintenance and future development of research at this institution.The SPECIFIC AIMS for improving this facility are: 1. to continue to acquire the molecular tools needed to analyze basic biomedical problems in a more detailed and modern fashion. (2). to sponsor ?hand-on? workshops in molecular biological techniques and protein expression and protein purification techniques given by invited experts of the fields. This will help the UCC researchers to solve their problem and to obtain preliminary data in their areas of their research. (3) to have the facility for the protein expression and purification in the UCC, and to encourage the UCC faculty members to expand their research to the protein level. (4) to continue and expand the resource centre where UCC faculty can obtain technical informations and cost estimations for molecular biological techniques and services which are not available in the UCC. (5) to continue and improve the computerized analysis of nucleic acid and protein data, and (6) to implement a ? fee for service? plan that will be used for the service contract/repair for VersaDOC system of Bio-RAD. This is to start to get partial self-support for the PNACF component of the RCMI program.