Location location location - how genome position affects gene expression

细菌基因组经过数十亿年的进化，使正确的基因在正确的时间高效表达。很明显，放在细菌染色体周围不同位置的同一个基因可以表现出非常不同的表达水平。然而，管理这一点的规则还没有完全理解。这个项目旨在研究基因组中的位置如何影响基因表达，这将帮助我们了解基因组是如何工作和进化的，并使我们能够合理设计合成染色体，从而实现重要基因的优化表达。该项目将比较沙门氏菌和大肠杆菌-这两个相关物种共享一些基因组组织，但控制基因表达的方式不同-的数百万个相同基因启动子副本的表达，使用工具，允许在不同条件下测量表达，以及分子微生物学、转录学和信息学的组合。

Bacterial genomes have evolved over billions of year to allow efficient expression of the right genes at the right times. It has become clear that the same gene, placed in different locations around a bacterial chromosome can display very different levels of expression. The rules which govern this are however not fully understood. This project aims to examine how position within a genome can affect gene expression, this will help us both understand how genomes work and evolve, and allow us to rationally design synthetic chromosomes which will deliver optimised expression of important genes. The project will compare expression of millions of copies of the same gene promoters in both Salmonella and E. coli - two related species which share some genome organisation but control gene expression differently - using tools to allow expression to be measured under different conditions, and a mix of molecular microbiology, transcriptomics and informatics.