HLA Region Genetics and SLE in U.S. Black Women

描述（由申请人提供）：系统性红斑狼疮（SLE）是一种慢性炎症性自身免疫性疾病，主要影响非裔美国女性。大量研究表明，HLA区域基因在SLE的遗传易感性中起着关键作用，但迄今为止的研究尚未确定HLA区域的哪些因素是重要的，以及HLA在多大程度上解释了总体风险。我们建议从黑人妇女健康研究（BWHS）中收集大量统一的样本来解决这些问题，BWHS是迄今为止对非洲裔美国妇女进行的最大的随访研究。我们将使用从400名患有SLE的BWHS参与者和800名匹配对照中收集的脸颊细胞样本。由于脸颊细胞样本的收集已经得到资助，目前的资助将集中在(a)样本的DNA提取，(b) DNA扩增，(c) HLA区域的SNP单倍型，(d)基于pcr的C4A缺失等位基因的基因分型，该等位基因与SLE有特殊的关联，以控制其影响，以及(e)一组SNP基因分型，以控制非洲裔美国人的欧洲混合。我们将详尽地调查HLA区域与SLE的遗传关联；这将涉及在该区域进行高密度snp的分型，这将为检测导致SLE风险增加2倍或更大的变异提供出色的能力。我们将评估的特定基因包括TNF、IKBL、MICA和HLA-DRB1，并对C4A基因缺失状态进行调整。此外，我们将在基因组中对一组snp进行基因分型，以确保我们检测到的任何与HLA等位基因的关联都是真实的，而不是由于群体分层（病例和对照组之间等位基因频率的系统性差异是由于其群体祖先的差异）。这也将使我们能够评估基因组的特定区域是否在非裔美国SLE患者中显示出异常高或低水平的欧洲血统，从而允许我们使用“混合作图”方法来寻找包含改变hla诱导SLE风险的基因的基因组部分。拟议的研究是基于人群的，不仅使用了一种创新的、统计学上强大的设计——它将详尽地调查HLA区域的SLE风险，并搜索基因组的其余部分以寻找风险的修饰因子——而且还将重点放在非裔美国女性身上，这是一个SLE的高风险人群，但在医学和科学上都得不到充分的服务。

DESCRIPTION (provided by applicant): Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease that disproportionately affects African-American women. Numerous studies have shown that HLA-region genes play a key role in genetic susceptibility to SLE, but studies so far have not established which factors in the HLA region are important and how much of overall risk is explained by HLA. We propose to address these questions with a large and uniformly collected group of samples from the Black Women's Health Study (BWHS), the largest follow-up study of African-American women yet conducted. We will use cheek cell samples collected from 400 BWHS participants with SLE and 800 matched controls. Since the collection of cheek cell samples is already funded, the present grant will focus on (a) DNA extraction from the samples, (b) DNA amplification, (c) SNP haplotyping of the HLA region, (d) PCR-based genotyping for the C4A deletion allele for which there is particular evidence of association to SLE in order to control for its effect, and (e) genotyping a panel of SNPs to control for European admixture in African-Americans. We will exhaustively survey the HLA region for genetic associations with SLE; this will involve typing a high-density panel of SNPs over the region, which will provide excellent power to detect variants that confer 2-fold or greater increased risk for SLE. Among the specific genes that we will assess are TNF, IKBL, MICA, and HLA-DRB1, with adjustment for C4A gene deletion status. In addition, we will genotype a panel of SNPs across the genome to ensure that any associations with HLA alleles that we detect are real rather than due to population stratification (systematic differences in allele frequency between cases and controls due to differences in their population ancestry). This will also allow us to assess whether specific regions of the genome show unusually high or low levels of European ancestry in African-American SLE patients, thus allowing us to use an 'admixture mapping' approach to find sections of the genome containing genes that modify HLA-induced risk for SLE. The proposed study is population-based and not only uses an innovative and statistically high-powered design-which will exhaustively survey the HLA region for SLE risk, as well as search the rest of the genome for modifiers of that risk-but also focuses on African-American women, a population that is high-risk for SLE but medically and scientifically underserved.