Photofragmentation MS of Ion Mobility Separated Peptides

描述（由申请人提供）：离子迁移率（IM）是一个完美的仪器平台，用于减缓和分离肽离子-这一创新使有效的VUV光致裂解（IM-oTOFMS/MS）在第一阶段得到了证明。VUV光致碎裂产生了一种近乎完美的技术，用于复杂肽混合物的“按需”结构碎片离子分析，特别是当与IM相结合时，其已被证明具有区分磷酸化肽与非磷酸化肽的能力。此外，lonwerks [34-37]最近内部资助的研究相当意外地证明了将纳米颗粒MALDI基质掺入生物组织近表面的功效。最近，lonwerks出版物[34-40]和专利[41-44]已经建立了一种新的方法来对生物组织表面进行分子微探针成像。因此，从颗粒处理的生物表面的复杂的激光微探针分子离子光谱的简化是另一个应用，这也将受益于离子迁移率分离的组合，然后按需光致碎裂结构分析。第二阶段将开发独特的IM-oTOFMS/MS硬件，在专门设计的oTOFMS中使用专利的多像素位置敏感探测器。增强质量和流动性的分辨率，以及该仪器的光碎片MS将进行测试，在蛋白质组学和组织成像的应用。商业化取决于显示只有IMS-oTOFMS/MS可以执行这些应用。

DESCRIPTION (provided by applicant): Ion mobility (IM) is a perfect instrumental platform for slowing and separating peptide ions-this innovation has enabled efficient VUV photofragmentation (IM-oTOFMS/MS) to be demonstrated in phase I. VUV photofragmentation yields a near perfect technology for "on demand" structural fragment ion analysis of complex peptide mixtures especially when combined with the proven capability of IM to distinguish phosphorylated vs. unphosphorylated peptides. In addition, recent internally funded research at lonwerks [34-37] has rather unexpectedly proved the efficacy of incorporating nanoparticulate MALDI matrices into the near surface of biotissues. Recently, lonwerks publications [34-40] and patents [41-44] have established a new approach to molecular microprobe imaging of biotissue surfaces. Thus, the simplification of complex laser microprobe molecular ion spectra from particulate treated biosurfaces is another application which will also benefit from the combination of ion mobility separation followed with on demand photofragmentation structural analysis. Phase II will develop unique IM-oTOFMS/MS hardware using patented multi-pixel position sensitive detectors in a specially designed oTOFMS. Enhanced mass and mobility resolution as well as photofragment MS of this instrument will be tested for applications in proteomics and tissue imaging. Commercialization depends on showing that only IMS-oTOFMS/MS can perform these applications.