MMPC: Diabetes and Diabectic Complications

总体描述(由申请人提供)： 华盛顿大学小鼠代谢表型中心的主要目标是促进和促进肥胖、糖尿病和由这些疾病引起的病理并发症的动物模型的研究。由于表型方法的多学科性质，医学、病理学和分子学科之间的密切相互作用对于描述用于研究人类疾病的动物是必要的。通过提供许多核心设施，西雅图MMPC整合和协调研究活动，以便有兴趣将其动物模型特色化的客户可以获得每种动物的新陈代谢和疾病状态的详细信息。这五个核心是：1)负责MMPC日常管理的管理核心，包括糖尿病并发症动物模型联合会(AMDCC)的试点和可行性计划；2)动物核心，将确保适当的动物交付和适当的分配到其他核心，并监测动物的健康、饲养和适当的治疗；3)糖尿病和能量平衡核心，提供测量身体成分、能量消耗、代谢表型和1型和2型糖尿病评估的设施；4)心血管核心，提供正常和挑战状态下的心功能评估、血管病理量化和对损伤建模的反应；5)微血管和视网膜病变核心，将评估肾脏功能和结构变化以及眼睛的病理程度。其中四个核心单元由集线器支持，这些集线器收集在每个集线器中的通用技术。在动物核心内是动物中心，该中心提供人员协助将动物分散到适当的核心设施，并协助调查人员进行表型鉴定。糖尿病和能量平衡核心包含分析中心，负责量化RNA、蛋白质、碳水化合物和MMPC所需的任何其他分析物。手术中心位于心血管核心内，为这一核心提供手术专业知识，并提供从其他核心延伸出来的任何手术需求。形态中心位于微血管/视网膜病变核心，负责所有组织的准备和分析。因此，这个MMPC为包括我们大学、AMDCC研究人员和全国其他潜在客户在内的庞大而多样的肥胖和糖尿病研究基地提供了出色的设施和支持。

DESCRIPTION, OVERALL (provided by applicant): The major goal of the Mouse Metabolic Phenotyping Center at the University of Washington is to promote and facilitate the study of animal models of obesity, diabetes and pathological complications due to these disorders. Because of the multidisciplinary nature of phenotyping approaches, close interaction across medical, pathological, and molecular disciplines is necessary to characterize animals used to study human diseases. By providing a number of Core facilities, the Seattle MMPC integrates and coordinates research activities so that Customers interested in having their animal models characterized can obtain a dear picture of the metabolic and disease state for each animal. The five Cores are: 1) an Administrative Core that is responsible for the day-to-day administration of the MMPC including the Pilot & Feasibility Program of the Animal Models of Diabetic Complications Consortium (AMDCC); 2) an Animal Core which will ensure proper delivery of animals and appropriate distribution to other Cores, and monitor health, husbandry and proper treatment of animals; 3) a Diabetes and Energy Balance Core to provide facilities for measuring body composition, energy expenditure, metabolic phenotyping and evaluation of type 1 and type 2 diabetes; 4) a Cardiovascular Core to provide assessment of heart function in normal and challenged states, vascular pathology quantifications, and response to injury modeling; 5) a Microvascular and Retinopathy Core which will evaluate kidney function and structural changes as well as extent of pathology in the eye. Four of the Core units are supported by HUBs which collect within each HUB, general technologies. Within the Animal Core is the Animal HUB which provides personnel to aid in dispersal of animals to the appropriate core facilities and to aid investigators in phenotyping. The Diabetes and Energy Balance Core harbors the Analytical HUB which is responsible for quantification of RNA, proteins, carbohydrates, and any other analyte needed in this MMPC. The Surgery HUB is within the Cardiovascular Core and provides surgery expertise for this Core and any surgery needs extending from other Cores. The Morphology HUB, located within the Microvascular/Retinopathy Core, is responsible for all tissue preparation and analysis. Thus, this MMPC provides outstanding facilities and support for the large and varied obesity and diabetes research base which includes our University, AMDCC investigators, and other potential Customers across the country.