Core F: Cell Separation & Sample Coordination Core

细胞分离和样品制备(CSSP)核心的总体目标是开发用于 适用于危重病人的浓缩血白细胞群的采集与分离 用于随后的高通量蛋白质组和基因组分析以及功能分析的群体 蛋白质组学。该中心还协助收集、处理和存档所获得的固体组织样本 在创伤和烧伤患者群体中进行外科干预。核心有三个主要功能 在该计划范围内：(1)开发新技术，从 用于随后的基因组和蛋白质组分析的危重创伤和烧伤患者，最初采用 宏观技术，但迅速转向微流体方法；(2)实施和支持 临床现场的宏观和微流控方案，包括质量控制和质量保证；以及 (3)血浆、白细胞和固体组织标本(RNA、蛋白质)的长期保存和存档，以备将来使用 分析。由于这些不同的功能，核心具有开发和服务组件。这个 主要服务地点在佛罗里达大学，样本收集试剂盒就是在那里生产的 在那里，样本被存档并储存在24小时监测的冷冻室和设施中。这个 主要开发地点在医学工程中心，那里有微流体盒 在罗切斯特大学和佛罗里达大学医学院 对宏观样品处理进行了开发和验证。

The overall goal of the Cell Separation and Sample Preparation (CSSP) Core is to develop technologies for the collection and isolation of enriched blood leukocyte cell populations that are applicable to critically ill patient populations for subsequent high throughput proteomic and genomic analyses, as well as for functional proteomics. The Core also assists with the collection, processing and archival of solid tissue samples obtained at surgical interventions in the trauma and burn patient populations. The Core has three primary functions within the Program: (1) the development of new technologies for the isolation of leukocyte subpopulations from critically-ill trauma and burn patients for subsequent genomic and proteomic analyses, initially employing macroscale techniques, but rapidly moving to a microfluidics approach; (2) the implementation and support of macroscale and microfluidics protocols at the clinical sites, including quality control and quality assurance; and, (3) the long-term storage and archival of plasma, leukocyte and solid tissue samples (RNA, protein) for future analyses. Because of these distinct functions, the Core has both development and service components. The primary service site is at the University of Florida where the Sample Collection Kits are manufactured and distributed, and where samples are archived and stored in 24-hour monitored freezers and facilities. The primary development sites are at the Center for Engineering in Medicine where the microfluidics cassettes are developed, and at the University of Rochester and the University of Florida College of Medicine where macroscale sample processing is developed and validated.