Biochemistry of Herpes Simplex Tegument Components

疱疹病毒是许多人类疾病的罪魁祸首。所有疱疹病毒都有一层称为被皮的蛋白质层，它排列在包膜的内表面。被皮和包膜的正确组装对于传染性病毒颗粒的产生至关重要，因此对于疾病的进展至关重要。然而，对于被膜蛋白如何识别和结合特定的细胞膜，以及它们如何确保它们组装成成熟的病毒粒子，人们知之甚少。本提案的第一部分将研究单纯疱疹病毒（HSV）蛋白vhs的生物化学，这是HSV被膜的一个组成部分。我们之前有过

The Herpes viruses are responsible for many human diseases. All Herpes viruses possess a proteinaceous layer termed tegument which lines the inner surface of their envelope. Correct assembly of tegument and envelope is essential for the production of an infectious viral particle, and thus for the progression of disease. However, little is known of how tegument proteins recognize and bind to specific cellular membranes, nor how they ensure their assembly into the maturing virion. The first part of this proposal will investigate the biochemistry of the Herpes simplex virus (HSV) protein vhs, a component of the HSV tegument. We have previously identified several biochemically distinct forms of vhs in infected cells. We will now investigate the relationship between these molecules and determine their importance for assembly of vhs into tegument. Next, we will dissect the molecular mechanism by which vhs binds to intracellular membranes. Finally, we have successfully isolated primary enveloped HSV particles from the perinuclear space. We will study the composition and function of tegument in these HSV assembly intermediates.