Project to be confirmed after rotation year

突触的突触前囊泡释放的神经递质允许神经元在大脑中进行交流。然而，不同功能类型的突触囊泡在突触内的详细空间排列仍然未知。我们将使用最先进的超分辨率显微镜，包括多色、实时和3DSTORM/STED显微镜，以及膨胀显微镜，以及开发新的计算图像分析方法来解决这个问题。然后，我们将使用这些方法来了解这种变化在突触发育过程中是如何变化的，对神经元活动的反应以及关键突触蛋白受到干扰的时候。

Neurotransmitter release from presynaptic vesicles at the synapse allows neurons tocommunicate in the brain. However the detailed spatial arrangement of differentfunctional types of synaptic vesicles within the synapse remains unknown. We will usestate-of-the-art super-resolution microscopy, including multi-colour, live and 3DSTORM/STED microscopy, and expansion microscopy, together with developing newcomputational image analysis approaches, to address this question. We will then usethese approaches to understand how this changes during synapse development, inresponse to neuronal activity and when key synaptic proteins are perturbed.