Agilent 6890 GC/5973 mass detector for Profiling

描述（由申请人提供）：我们是一组长期合作的研究人员，我们对代谢疾病和动脉粥样硬化有共同的兴趣。戴维斯实验室对该小组技术专长的贡献是使用气相/液相色谱法和GC/质谱法提供脂质定量和氧化甾醇分析。本申请的目的是寻求资金来购买GC/质谱仪，使我们能够分析细胞，组织和分离物的脂质，固醇和代谢物。此外，这台机器将使我们能够进行稳定的同位素稀释分析，以评估表型（即脂质，固醇和代谢物的体内代谢）如何与特定小鼠的遗传背景相关联。我们要求使用安捷伦6890气相色谱，该气相色谱具有与火焰电离检测器连接的可分裂/不可分裂全玻璃注射器，以及5973质量选择检测器，使我们能够定量胆固醇/甾醇/脂肪酸，并分析基因和基因改变小鼠的代谢谱，作为将基因组学与代谢表型（重点是动脉粥样硬化易感性）联系起来的手段。该设备需要取代戴维斯实验室19年的惠普5890A气相色谱，该气相色谱具有连接火焰电离检测器和5970质量选择检测器的分裂/无分裂全玻璃注入器。在过去的3年里，戴维斯实验室花费了8000多美元来使用回收的零件反复固定这台仪器。不幸的是，这是浪费时间和金钱，因为机器的许多零件都过时了，无法更换。我们现在不得不同意安捷伦的意见，即我们的旧机器不能以可靠的方式修理。正是出于这个原因，我们寻求资金购买一台替换的GC/质谱仪。各实验室使用了GC甾醇分析，如表1所示。由于我们对这种技术的依赖，在分解之前，我们使用GC -60%的时间。每个研究者的预计使用情况如表1所示，将占100%的时间使用。因此，对于这项重要的技术，我们没有替代设备。

DESCRIPTION (provided by applicant): We are a group of investigators with long-term collaborations centering on our common interest in metabolic disease and atherosclerosis. The Davis lab's contribution to the technical expertise of the group has been to provide lipid quantitation and oxysterol analysis using gas/liquid chromatography and GC/mass spectroscopy. The purpose of this application is to seek funds to procure a GC/mass spectrometer allowing us to analyze cells, tissues and isolates for lipids, sterols and metabolites. In addition, this machine will enable us to perform stable isotope dilution analysis in order to assess how phenotype (i.e. the in vivo metabolism of lipids, sterols and metabolites) may be linked to the genetic background of specific mice. We request an Agilent 6890 GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5973 mass selective detector enabling us to quantitate cholesterol/sterols/fatty acids and to analyze the metabolic profiles of congenic and genetically altered mice as a means to link genomics with metabolic phenotypes with emphasis on susceptibility to atherosclerosis. This equipment is needed to replace the Davis lab's 19 year old Hewlett-Packard 5890A GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5970 mass selective detector. During the past 3 years, the Davis lab has spent over $8000 to have this instrument repeatedly fixed using salvaged parts. Unfortunately, this was a waste of time and money, as many parts to the machine were outdated and replacement not possible. We have now have been forced to agree with Agilent that our old machine can not be fixed in a reliable manner. It is for this reason that we seek funds to purchase a replacement GC/mass spectrometer. Each lab has used GC sterol analysis as shown in table 1. Because of our dependence on this technique, prior to breaking down we used the GC -60% of the time. The projected use of each investigator is shown in table 1 and will account for 100% time use. Therefore, we have no alternative equipment for this essential technique.