LSR II ANALYZER

描述（由申请人提供）： 本申请要求为华盛顿大学的现有共享资源细胞分析设施（CAF）提供BD LSRII流式细胞仪。CAP由免疫学系于1991年建立，自那时以来一直在持续运作。在过去的一年中，来自医学院28个不同部门和其他8所学校或学院以及区域灵长类动物中心的100多名主要研究人员的实验室工作人员使用了该设施。这些研究人员跨越了非常多样化的生物医学和生物学研究兴趣，并享受来自NIH和其他联邦机构的强大资金。本申请记录了我们目前的设施在满足这些研究人员对现代流式细胞仪的需求方面的局限性，以及LSR II分析仪如何使我们能够满足这些需求。

DESCRIPTION (provided by applicant): This application requests a BD LSRII flow cytometric analyzer for an existing shared resource Cell Analysis Facility (CAF) at the University of Washington. The CAP was established by the Department of Immunology in 1991 and has been in continuous operation since that time. During the past year, research personnel working in the laboratories of more than 100 principal investigators from 28 different departments in the School of Medicine and 8 other Schools or Colleges and the Regional Primate Center used the facility. These investigators span an extraordinarily diverse range of biomedical and biological research interests and enjoy robust funding from the NIH and other federal agencies. This application documents the limitations of our current facility in meeting the needs of these investigators for access to contemporary flow cytometry instruments and how an LSR II analyzer would allow us to meet these needs.