PRESENTATIONS BY CRISTIAN T BADEA

描述(由申请人提供)： 利用小动物进行基础生物医学研究的巨大增长催生了对啮齿动物结构和功能表型新方法的迫切需求。我们建议继续建立杜克活体显微镜综合中心，这是NIH/NCRR的一个国家资源，明确地专注于满足这一需求。我们提出了四个集成的核心，将为来自世界各地的研究人员提供新的基于图像的方法，使用磁共振显微镜(MRM)进行结构和功能表型鉴定。核心A：我们将在啮齿类动物中开发功能显微镜，以支持心脏运动分析、使用动脉自旋标记的肺灌注和使用超极化气体成像的肺通风。核心B：我们将开发更高效的编码策略，将MR组织学的吞吐量提高至少500%。我们将开发用于3D MR组织学的固定组织的主动染色方法。我们将开发方法来测量小鼠大脑中的局部神经退化。核心C：我们将开发新一代小动物的生理支持和监测技术，允许多峰成像(MRM)。MicroPET、microCT等)以及对免疫系统受损的动物进行图像配准和长期研究。核心D：我们将开发视觉信息学工具，以便有效地存档、访问和分析来自多个医疗模式的非常大的多维图像集。这些技术将支持总共16名合作者，他们急于使用小动物成像来扩展从癌症到神经生物学的各种主题的视野。一个积极的教育和传播计划，将包括本科生和研究生，以及博士后研究员。

DESCRIPTION (provided by applicant): The enormous growth in the use of small animals for basic biomedical research has created a compelling need for new approaches to structural and functional phenotyping in the rodent. We propose continuation of the Duke Integrated Center for In Vivo Microscopy, an NIH/NCRR National Resource with an explicit focus on meeting this need. We propose four integrated cores that will provide researchers from around the world new image-based methods for structural and functional phenotyping using magnetic resonance microscopy (MRM). Core A: We will develop functional microscopy in the rodent to support cardiac motion analysis, pulmonary perfusion using arterial spin tagging and pulmonary ventilation using hypcrpolarized gas imaging. Core B: We will develop more efficient encoding strategies to increase our throughput for MR Histology by at least 500%. We will develop methods for actively staining fixed tissues for 3D MR histology. We will develop methods to measure regional neurodegeneration in the mouse brain. Core C: We will develop a new generation of technologies for physiologic support and monitoring of small animals permitting mutlimodality imaging (MRM. MicroPET, microCT, etc.) and image registration and long term studies on animals with compromised immunologic systems. Core D: We will develop visual informatics tools to permit effective archive, access, and analysis of very large multidimensional image sets from multiple modalities. The technologies will support a total of 16 collaborators anxious to use small animal imaging to extend the horizons of topics ranging from cancer to neurobiology. An aggressive education and dissemination program is included that will include undergraduate and graduate students, as well as post-doctoral fellows.